CHARACTERIZATION AND PHYLOGENY OF MCRII, A GENE-CLUSTER ENCODING AN ISOENZYME OF METHYL COENZYME-M REDUCTASE FROM HYPERTHERMOPHILIC - METHANOTHERMUS-FERVIDUS

被引:25
作者
LEHMACHER, A [1 ]
KLENK, HP [1 ]
机构
[1] MAX PLANCK INST BIOCHEM,D-82152 MARTINSRIED,GERMANY
来源
MOLECULAR & GENERAL GENETICS | 1994年 / 243卷 / 02期
关键词
METHANOGEN; METHYL COENZYME M REDUCTASE; PHYLOGENY; PROMOTER; TRANSCRIPTION TERMINATION;
D O I
10.1007/BF00280317
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A 5.7 kb region of chromosomal DNA from Methanothermus fervidus, harbouring a second mcr gene cluster, was cloned and sequenced. This gene cluster, termed mcrII, encodes an isoenzyme of methyl coenzyme M reductase (MCR). In contrast to the known mcr gene clusters from other methanogens, mcrII lacks mcrC, a gene of unknown function. But the remaining mcrII genes B, D, G and A are arranged in the same order as in previously sequenced mcr gene clusters. The mcrII genes from M. fervidus are located 3' to the open reading frame (ORF) B of the methylviologen-reducing hydrogenase (mvh) gene cluster. The genes of mcrII are cotranscribed, resulting in an mRNA of 4500 nucleotides. The transcriptional initiation and termination sites were identified. Phylogenetic reconstructions reveal that the mcr gene clusters fall into two different types, I and II, and that the ancestral mcr gene cluster was duplicated before the segregation of methanogens into three major orders occurred.
引用
收藏
页码:198 / 206
页数:9
相关论文
共 32 条
[1]   NUCLEOTIDE-SEQUENCE OF THE METHYL COENZYME-M REDUCTASE GENE-CLUSTER FROM METHANOSARCINA-BARKERI [J].
BOKRANZ, M ;
KLEIN, A .
NUCLEIC ACIDS RESEARCH, 1987, 15 (10) :4350-4351
[2]   CLONING AND CHARACTERIZATION OF THE METHYL COENZYME-M REDUCTASE GENES FROM METHANOBACTERIUM-THERMOAUTOTROPHICUM [J].
BOKRANZ, M ;
BAUMNER, G ;
ALLMANSBERGER, R ;
ANKELFUCHS, D ;
KLEIN, A .
JOURNAL OF BACTERIOLOGY, 1988, 170 (02) :568-577
[3]   DIFFERENTIAL EXPRESSION OF THE 2 METHYL-COENZYME M-REDUCTASES IN METHANOBACTERIUM-THERMOAUTOTROPHICUM AS DETERMINED IMMUNOCHEMICALLY VIA ISOENZYME-SPECIFIC ANTISERA [J].
BONACKER, LG ;
BAUDNER, S ;
THAUER, RK .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1992, 206 (01) :87-92
[4]   STRUCTURE AND EXPRESSION OF THE GENES, MCRBDCGA, WHICH ENCODE THE SUBUNITS OF COMPONENT-C OF METHYL COENZYME-M REDUCTASE IN METHANOCOCCUS-VANNIELII [J].
CRAM, DS ;
SHERF, BA ;
LIBBY, RT ;
MATTALIANO, RJ ;
RAMACHANDRAN, KL ;
REEVE, JN .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (12) :3992-3996
[5]  
Dayhoff MO, 1978, ATL PROTEIN SEQ STRU, V5, P345
[6]  
Efron B., 1982, CBMS NSF REGIONAL C
[7]   THE FINAL STEP IN METHANE FORMATION - INVESTIGATIONS WITH HIGHLY PURIFIED METHYL-COM REDUCTASE (COMPONENT-C) FROM METHANOBACTERIUM-THERMOAUTOTROPHICUM (STRAIN MARBURG) [J].
ELLERMANN, J ;
HEDDERICH, R ;
BOCHER, R ;
THAUER, RK .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1988, 172 (03) :669-677
[8]  
FELSENSTEIN J, 1991, PHYLIP USERS MANUAL
[9]   ALIGNING AMINO-ACID SEQUENCES - COMPARISON OF COMMONLY USED METHODS [J].
FENG, DF ;
JOHNSON, MS ;
DOOLITTLE, RF .
JOURNAL OF MOLECULAR EVOLUTION, 1985, 21 (02) :112-125
[10]   CONSTRUCTION OF PHYLOGENETIC TREES [J].
FITCH, WM ;
MARGOLIASH, E .
SCIENCE, 1967, 155 (3760) :279-+