AUTORADIOGRAPHIC LOCALIZATION AND ANALYSIS OF ENDOTHELIN-1 BINDING-SITES IN HUMAN SYNOVIAL TISSUE

被引：26

作者：

WHARTON, J ^{[1
]}

RUTHERFORD, RAD ^{[1
]}

WALSH, DA ^{[1
]}

MAPP, PI ^{[1
]}

KNOCK, GA ^{[1
]}

BLAKE, DR ^{[1
]}

POLAK, JM ^{[1
]}

机构：

[1] LONDON HOSP,INFLAMMAT GRP,LONDON E1 1BB,ENGLAND

来源：

ARTHRITIS AND RHEUMATISM | 1992年 / 35卷 / 08期

关键词：

D O I：

10.1002/art.1780350807

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Objective. To determine the localization of endothelin binding sites and immunoreactivity in human synovial tissues. Methods. Quantitative in vitro autoradiographic and immunohistochemical techniques were used to localize and characterize I-125-labeled endothelin-1 (I-125-ET-1) binding sites and endothelin-like immunoreactivity in sections of rheumatoid, osteoarthritic, and normal synovium. Results. Specific I-125-ET-1-binding sites, characteristic of the ET(A) receptor, were localized to the media of synovial blood vessels in all 3 groups. No difference was found in vascular binding site density in rheumatoid and osteoarthritic synovium. Endothelin-like immunoreactivity was localized to endothelial cells in blood vessels displaying I-125-ET-1 binding sites. Conclusion. We conclude that endothelin may act locally, modulating synovial perfusion and exacerbating hypoxia in chronic arthritis.

引用

页码：894 / 899

页数：6

共 13 条

[1] CLONING AND EXPRESSION OF A CDNA-ENCODING AN ENDOTHELIN RECEPTOR [J].