PRODUCTION OF EXTRACELLULAR EMULSIFYING AGENT BY PSEUDOMONAS-AERUGINOSA UG1

被引：24

作者：

MACELWEE, CG ^{[1
]}

LEE, H ^{[1
]}

TREVORS, JT ^{[1
]}

机构：

[1] UNIV GUELPH,DEPT ENVIRONM BIOL,GUELPH N1G 2W1,ONTARIO,CANADA

来源：

JOURNAL OF INDUSTRIAL MICROBIOLOGY | 1990年 / 5卷 / 01期

关键词：

Bacteria; Emulsifier; Oil; Pseudomonas aeruginosa;

D O I：

10.1007/BF01569603

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Twenty-three bacterial strains were isolated from oil-contaminated soil samples. Of these, 20 displayed some ability to effect oil dispersion and they were screened quantitatively for the ability to emulsify 0.5% (v/v) reference oil. One strain, identified as Pseudomonas aeruginosa UG1, produced extracellular material that emulsified reference oil, hexadecane and 2-methylnaphthalene at concentrations as high as 6% (v/v) in nutrient broth. Emulsification activity increased during a 10 day incubation period at 30°C. The activity was not influenced by pH over the range 5 to 9. The emulsifying agent was precipitated by cold ethanol. The highest emulsifying activity was detected in the extracellular fraction precipitated between 30 and 50% (v/v) ethanol. A linear relationship was observed between emulsifier concentration (mg/ml) and emulsifying activity. Genetic analysis showed that the Pseudomonas aeruginosa UG1 strain did not carry extrachromosomal plasmids, suggesting that the gene(s) coding for emulsifying activity was carried on the chromosome. © 1990 Society for Industrial Microbiology.

引用

页码：25 / 32

页数：8

共 26 条

[11]

Kaplan N., Zosim Z., Rosenberg E., Reconstitution of emulsifying activity of Acinetobacter calcoaceticus BD4 emulsan by using pure polysaccharide and protein, Appl. Environ. Microbiol., 53, pp. 440-446, (1987)

[12]

Kenne L., Lindberg B., Bacterial polysaccharides, The Polysaccharides, vol. 2, pp. 287-363, (1983)

[13]

Lennox E.S., Transduction of linked genetic characters of the host by bacteriophage P1, Virology, 1, pp. 190-206, (1955)

[14]

Mattei G., Bertrand J.C., Production of biosurfactants by a mixed bacteria population grown in continuous culture in crude oil, Biotechnol. Lett., 7, pp. 217-222, (1985)

[15]

Parkinson M., Bio-surfactants, Biotechnol. Adv., 3, pp. 65-83, (1985)

[16]

Persson A., Molin G., Capacity for biosurfactant production of environmental Pseudomonas and Vibrionaceae growing on carbohydrates, Applied Microbiology and Biotechnology, 26, 439, (1987)

[17]

Reddy P.G., Singh H.D., Roy P.K., Baruah J.N., Predominant role of hydrocarbon solubilization in the microbial uptake of hydrocarbons, Biotechnology and Bioengineering, 24, pp. 1241-1269, (1982)

[18]

Reddy P.G., Singh H.D., Pathak M.G., Bhagat S.D., Baruah J.N., Isolation and functional characterization of hydrocarbon emulsifying and solubilizing factors produced by a Pseudomonas species, Biotechnol. Bioengin., 19, pp. 1303-1320, (1983)

[19]

Rosenberg M., Baijer E.A., Delarea J., Rosenberg E., Role of thin fimbriae in adherence and growth of Acinetobacter calcoaceticus RAG-1 on hexadecane, Appl. Environ. Microbiol., 44, pp. 929-937, (1982)

[20]

Rosenberg E., Perry A., Gibson D.T., Gutnick D.L., Emulsifier of Arthrobacter RAG-1: Specificity of hydrocarbon substrate, Appl. Environ. Microbiol, 37, pp. 409-413, (1979)

← 1 2 3 →