EFFICIENT ELECTROPULSE TRANSFORMATION OF INTACT CANDIDA-MALTOSA CELLS BY DIFFERENT HOMOLOGOUS VECTOR PLASMIDS

被引：16

作者：

KASUSKE, A ^{[1
]}

WEDLER, H ^{[1
]}

SCHULZE, S ^{[1
]}

BECHER, D ^{[1
]}

机构：

[1] UNIV GREIFSWALD, FACHRICHTUNG BIOL, INST GENET, FL JAHNSTR 15, O-2200 GREIFSWALD, GERMANY

来源：

YEAST | 1992年 / 8卷 / 09期

关键词：

CANDIDA-MALTOSA; ELECTROPORATION; TRANSFORMATION; PLASMID VECTORS;

D O I：

10.1002/yea.320080902

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Conditions for efficient and quick transformation by electroporation were developed in Candida maltosa. To investigate the efficiency of transformation with integrative as well as with autonomously replicating plasmids, a series of vectors was constructed for homologous transformation of this species. Transformants were obtained with different plasmids as covalently closed circular molecules and as linearized DNA. The influence of recipient strain and plasmid type as well as of cell number and parameters of the supplied electrical pulse on the transformation efficiency have been investigated. A maximum of 7000 transformants per 100 ng of plasmid DNA was reached. The efficiency of transformation was compared with that of the LiCl method.

引用

页码：691 / 697

页数：7

共 14 条

[1] HIGH-FREQUENCY TRANSFORMATION OF THE FISSION YEAST SCHIZOSACCHAROMYCES-POMBE [J].

BEACH, D ;

NURSE, P .

NATURE, 1981, 290 (5802) :140-142

[2] CORRELATION OF BIOCHEMICAL BLOCKS AND GENETIC LESIONS IN LEUCINE AUXOTROPHIC STRAINS OF THE IMPERFECT YEAST CANDIDA-MALTOSA [J].

BECHER, D ;

WEDLER, H ;

SCHULZE, H ;

BODE, R ;

KASUSKE, A ;

SAMSONOVA, I .

MOLECULAR AND GENERAL GENETICS, 1991, 227 (03) :361-368

[3]

BECKER DM, 1991, METHOD ENZYMOL, V194, P182

[4] TRANSFORMATION OF YEAST BY A REPLICATING HYBRID PLASMID [J].

BEGGS, JD .

NATURE, 1978, 275 (5676) :104-109

[5] STERILE HOST YEASTS (SHY) - EUKARYOTIC SYSTEM OF BIOLOGICAL CONTAINMENT FOR RECOMBINANT DNA EXPERIMENTS [J].

BOTSTEIN, D ;

FALCO, SC ;

STEWART, SE ;

BRENNAN, M ;

SCHERER, S ;

STINCHCOMB, DT ;

STRUHL, K ;

DAVIS, RW .

GENE, 1979, 8 (01) :17-24

[6]

BROACH JR, 1979, GENE, V8, P121, DOI 10.1016/0378-1119(79)90012-X

[7] A HIGH-FREQUENCY TRANSFORMATION SYSTEM FOR THE YEAST KLUYVEROMYCES-LACTIS [J].

DAS, S ;

HOLLENBERG, CP .

CURRENT GENETICS, 1982, 6 (02) :123-128

[8] TRANSFORMATION OF SACCHAROMYCES-CEREVISIAE BY ELECTROPORATION [J].

DELORME, E .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1989, 55 (09) :2242-2246

[9] AN IMPROVED HOST-VECTOR SYSTEM FOR CANDIDA-MALTOSA USING A GENE ISOLATED FROM ITS GENOME THAT COMPLEMENTS THE HIS5 MUTATION OF SACCHAROMYCES-CEREVISIAE [J].

HIKIJI, T ;

OHKUMA, M ;

TAKAGI, M ;

YANO, K .

CURRENT GENETICS, 1989, 16 (04) :261-266

[10] TRANSFORMATION OF YEAST [J].

HINNEN, A ;

HICKS, JB ;

FINK, GR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (04) :1929-1933

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