THE FAT-BODY CELL-FREE SYSTEM FOR TISSUE-SPECIFIC TRANSCRIPTION OF PLASMA-PROTEIN GENE OF BOMBYX-MORI

被引:13
作者
MINE, E
SAKURAI, H
IZUMI, S
TOMINO, S
机构
[1] TOKYO METROPOLITAN UNIV,DEPT BIOL,HACHIOJI,TOKYO 19203,JAPAN
[2] KEIO UNIV,SCH MED,GENET MOLEC LAB,SHINJUKU KU,TOKYO 160,JAPAN
关键词
D O I
10.1093/nar/23.14.2648
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A nuclear extract was prepared from the larval fat body of the silkworm, Bombyx mori, and a homologous in vitro system was developed for the transcription of major plasma protein gene of B.mori, The gene for SP1, a storage protein of B.mori, and adenovirus 2 major late (AdML) gene were faithfully transcribed under relatively high template concentrations in the nuclear extract prepared from the fat body of female fifth instar larvae, Complete inhibition of gene transcription by a low concentration of alpha-amanitin indicated that the reaction is catalyzed by RNA polymerase II. At low template concentration (0.6 nM) the fat body nuclear extract transcribed the homologous SP1 gene with high efficiency, while AdML gene and larval cuticle protein gene were only barely transcribed in the same extract. The SP1 gene deleted upstream of the TATA box sequence showed little effect on transcription, whereas mutations that destroy TATA sequence totally abolished the gene transcription, These results suggested that the core promoter region of SP1 gene spanning between positions -44 and +16 is essential for the fat body specific transcription in vitro.
引用
收藏
页码:2648 / 2653
页数:6
相关论文
共 30 条
[21]   CHARACTERIZATION OF THE HUMAN CD4 GENE PROMOTER - TRANSCRIPTION FROM THE CD4 GENE CORE PROMOTER IS TISSUE-SPECIFIC AND IS ACTIVATED BY ETS PROTEINS [J].
SALMON, P ;
GIOVANE, A ;
WASYLYK, B ;
KLATZMANN, D .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (16) :7739-7743
[22]  
SUZUKI T, 1988, J BIOL CHEM, V263, P5979
[23]  
SUZUKI Y, 1990, DEV GROWTH DIFFER, V32, P179
[24]   TISSUE-SPECIFIC TRANSCRIPTION ENHANCEMENT OF THE FIBROIN GENE CHARACTERIZED BY CELL-FREE SYSTEMS [J].
SUZUKI, Y ;
TSUDA, M ;
TAKIYA, S ;
HIROSE, S ;
SUZUKI, E ;
KAMEDA, M ;
NINAKI, O .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (24) :9522-9526
[25]  
TAKIYA S, 1993, DEV GROWTH DIFFER, V35, P311
[26]   A CONTRIBUTION OF THE CORE-PROMOTER AND ITS SURROUNDING REGIONS TO THE PREFERENTIAL TRANSCRIPTION OF THE FIBROIN GENE IN POSTERIOR SILK GLAND EXTRACTS [J].
TAKIYA, S ;
HUI, CC ;
SUZUKI, Y .
EMBO JOURNAL, 1990, 9 (02) :489-496
[27]   CORE PROMOTER OF THE MOUSE MYELIN BASIC-PROTEIN GENE GOVERNS BRAIN-SPECIFIC TRANSCRIPTION INVITRO [J].
TAMURA, T ;
SUMITA, K ;
HIROSE, S ;
MIKOSHIBA, K .
EMBO JOURNAL, 1990, 9 (10) :3101-3108
[28]   TISSUE-SPECIFIC INVITRO TRANSCRIPTION FROM THE MOUSE MYELIN BASIC-PROTEIN PROMOTER [J].
TAMURA, TA ;
AOYAMA, A ;
INOUE, T ;
MIURA, M ;
OKANO, H ;
MIKOSHIBA, K .
MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (07) :3122-3126
[29]   CELL-SPECIFIC EXPRESSION OF THE HUMAN GASTRIN GENE - EVIDENCE FOR A CONTROL ELEMENT LOCATED DOWNSTREAM OF THE TATA BOX [J].
THEILL, LE ;
WIBORG, O ;
VUUST, J .
MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (12) :4329-4336
[30]   FAITHFUL TRANSCRIPTION INITIATION OF FIBROIN GENE IN A HOMOLOGOUS CELL-FREE SYSTEM REVEALS AN ENHANCING EFFECT OF 5' FLANKING SEQUENCE FAR UPSTREAM [J].
TSUDA, M ;
SUZUKI, Y .
CELL, 1981, 27 (01) :175-182