共 21 条
[21]
YVANYI L, 1977, CLIN IMMUNOL IMMUNOP, V7, P123
NON-SELECTIVE LYMPHOCYTE ISOLATION FROM HUMAN-BLOOD BY NYLON WOOL FILTRATION AND DENSITY CENTRIFUGATION
被引：10
作者：
DEPAUW, BE
WESSELS, JMC
GEESTMAN, EJM
SMEULDERS, JBJM
WAGENER, DJT
HAANEN, C
机构：
[1] Division of Hematology, Department of Internal Medicine, St. Radboud Hospital, Nijmegen
关键词：
D O I：
10.1016/0022-1759(79)90117-0
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ;
081704 ;
摘要：
A new method was employed to isolate lymphocytes from human peripheral blood. Continuous flow filtration through a nylon wool filter, at a flow rate of 1.4 ml/min, produced a lymphocyte yield of 90.5% and a purity of 96% without any shift in the B-T cell ratio. Ficoll-Isopaque with a specific gravity of 1.085 g/ml instead of 1.077 g/ml could be used to remove the erythrocytes. An overall recovery, including defibrination, filtration, Ficoll-Isopaque centrifugation and washing step, of 74.5% was achieved. © 1979.
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页码：291 / 295
页数：5
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