O-17 NMR AND FT-IR STUDY OF THE IONIZATION STATE OF PEPTIDES IN APROTIC-SOLVENTS - APPLICATION TO LEU-ENKEPHALIN

被引：9

作者：

GEROTHANASSIS, IP

BIRLIRAKIS, N

KARAYANNIS, T

TSIKARIS, V

SAKARELLOSDAITSIOTIS, M

SAKARELLOS, C

VITOUX, B

MARRAUD, M

机构：

[1] UNIV IOANNINA,ORGAN CHEM & BIOCHEM SECT,POB 1186,GR-45110 IOANNINA,GREECE

[2] INST NATL POLYTECH LORRAINE,ECOLE NATL SUPER IND CHIM,CNRS,URA 494,MACROMOLEC PHYS CHEM LAB,F-54001 NANCY,FRANCE

来源：

FEBS LETTERS | 1992年 / 298卷 / 2-3期

关键词：

FT-IR; IONIZATION STATE OF PEPTIDE; LEU-ENKEPHALIN; O-17; NMR;

D O I：

10.1016/0014-5793(92)80053-J

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The ionization state of Leu-enkephalin in DMSO and MeCN/DMSO (4/1) solution was studied by the combined use of O-17 NMR and FT-IR spectroscopy. After lyophilization of an aqueous solution at nearly neutral pH. Leu-enkephalin essentially exists in the uncharged state in MeCN/DMSO (4/1) solution. In pure DMSO, only 40% of the Leu-enkephalin molecules are in the zwitterionic state under the same conditions.

引用

页码：188 / 190

页数：3