ROLE OF ANTIBODY-SYNTHESIS AND COMPLEMENT ACTIVATION IN CONCORDANT XENOGRAFT RETRANSPLANTATION

被引:22
作者
GANNEDAHL, G
KARLSSONPARRA, A
WALLGREN, AC
ROOSENGSTRAND, E
NILSSON, B
TOTTERMAN, TH
TUFVESON, G
机构
[1] UNIV UPPSALA,DEPT TRANSPLANTAT SURG,S-75185 UPPSALA,SWEDEN
[2] GOTHENBURG UNIV,DEPT SURG,S-41345 GOTHENBURG,SWEDEN
关键词
D O I
10.1097/00007890-199408150-00015
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
A mouse-to-rat heart retransplantation model was used to study the effects of complement depletion and antibody production with regard to graft survival and anti-donor antibody specificity. Retransplantation was performed 3 weeks after the first transplantation in the presence or absence of 15-deoxyspergualin (DSG) immunosuppression. Untreated animals rejected their first graft after 3 days and retransplantation resulted in a hyperacute rejection within 2 min. A low titer of preformed anti-mouse lymphocytotoxic antibodies of the IgM subclass was found in serum collected from the unoperated rat. The rejection gave rise to a synthesis of IgG antidonor antibodies reacting with both graft endothelium and sarcolemma. Immunofluorescent staining of the rejected first heart graft showed moderate IgM and IgG antibody deposits on the graft vascular endothelium, while only IgG was found in the second graft. There was no C3 deposition found in the first mouse graft, as was the case in the second mouse graft. Anti-mouse antibodies cross-reacted with hamster antigens and a hyperacute rejection of a hamster heart graft occurred in a mouse-sensitized rat. Immunofluorescent staining revealed that the antibodies did not bind to hamster heart endothelium, as was expected, but, instead, to graft sarcolemma. DSG treatment prolonged the survival of the first graft by a median of 8 days. Continuous treatment until retransplantation resulted in a prolongation to 30 (20-127) min of the survival of the second graft and no increase in antibody titers against mouse antigens was observed. However, immunofluorescent staining revealed a weak binding of anti-mouse antibodies of the IgM subclass in the rejected mouse heart graft. Additional complement depletion with cobra venom factor in DSG-treated animals resulted in a prolongation of the median graft survival to 48 hr (6-96). No sign or minimal signs of antibody deposition were found in these grafts, but histology revealed massive mononuclear infiltration. In conclusion, xenograft transplantation in a concordant situation results in a shift of antidonor antibody Ig synthesis from IgM to IgG. If daily DSG treatment is administered from the day of transplantation, this reduces the synthesis of antidonor antibodies, and if complement is also depleted, the survival of the second graft is prolonged. The significance of the mononuclear infiltration remains to be established.
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页码:337 / 344
页数:8
相关论文
共 27 条
[1]  
BACH FH, 1992, TRANSPLANT P, V24, P739
[2]  
BALNER H., 1967, TRANSPLANTATION, V5, P624, DOI 10.1097/00007890-196707000-00007
[3]  
BOUWMAN E, 1989, TRANSPLANT P, V21, P551
[4]  
CALNE RY, 1970, TRANSPL P, V2, P550
[5]   CHARACTERIZATION OF THE PREFORMED ANTIBODIES INVOLVED IN THE XENOGRAFT REACTION [J].
CAMERON, DJ ;
RAJAGOPALAN, PR ;
FITTS, CT ;
MAJESKI, JA .
JOURNAL OF SURGICAL ONCOLOGY, 1983, 22 (03) :157-163
[6]   DECREASE OF MONONUCLEAR PHAGOCYTE CELL FUNCTIONS AND PROLONGATION OF GRAFT-SURVIVAL IN EXPERIMENTAL TRANSPLANTATION BY (+/-)-15-DEOXYSPERGUALIN [J].
DICKNEITE, G ;
SCHORLEMMER, HU ;
SEDLACEK, HH .
INTERNATIONAL JOURNAL OF IMMUNOPHARMACOLOGY, 1987, 9 (05) :559-565
[7]  
FORBES RDC, 1978, LAB INVEST, V39, P463
[8]   DEOXYSPERGUALIN DIRECTLY SUPPRESSES ANTIBODY-FORMATION INVIVO AND INVITRO [J].
FUJII, H ;
TAKADA, T ;
NEMOTO, K ;
YAMASHITA, T ;
ABE, F ;
FUJII, A ;
TAKEUCHI, T .
JOURNAL OF ANTIBIOTICS, 1990, 43 (02) :213-219
[9]   CHARACTERISTICS OF MOUSE TO RAT XENOGRAFT HEART-TRANSPLANTATION [J].
GANNEDAHL, G ;
FELLSTROM, B ;
LARSSON, E ;
ROOSENGSTRAND, E ;
TUFVESON, G .
EUROPEAN SURGICAL RESEARCH, 1990, 22 (04) :206-212
[10]   REJECTION ASSOCIATED WITH EARLY APPEARANCE OF DONOR-REACTIVE ANTIBODIES AFTER KIDNEY-TRANSPLANTATION TREATED WITH PLASMAPHERESIS AND ADMINISTRATION OF 15-DEOXYSPERGUALIN - A REPORT OF 2 CASES [J].
GANNEDAHL, G ;
OHLMAN, S ;
PERSSON, U ;
GUDMUNDSSON, S ;
LARSSON, E ;
TYDEN, G ;
TOTTERMAN, TH ;
WIKSTROM, B ;
WEISS, L ;
GROTH, CG ;
TUFVESON, G .
TRANSPLANT INTERNATIONAL, 1992, 5 (04) :189-192