FLUOROMETRIC ASSAY OF ALPHA-METHYLENE CARBONYL-COMPOUNDS WITH N1-METHYLNICOTINAMIDE CHLORIDE

被引：31

作者：

NAKAMURA, H

TAMURA, Z

机构：

[1] Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Hongo 7-3-1, Bunkyo-ku

来源：

ANALYTICAL CHEMISTRY | 1978年 / 50卷 / 14期

关键词：

D O I：

10.1021/ac50036a027

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A spectrophotofluorometric method has been developed for the determination of α-methylene carbonyl compounds by using N1-methylnicotinamide chloride (NMN). When reacted at 0 °C for 10 min with NMN in the presence of alkali and heated at 92 °C for 3 min after the addition of formic acid solution, only compounds having an α-methylene carbonyl group (-CH2-CO-) gave intense violet to green fluorescence. By measuring the fluorescence, as little as 5 pmol of ace-tophenone, 1-indanone and α-tetralon could be determined. The relative standard deviations of 1.00% and 1.61% are observed for the analyses of 1 X 10ˉ 9 mol and 1 X 10ˉ10 mol of acetophenone, respectively. The mechanism associated with the reaction is also discussed. © 1978, American Chemical Society. All rights reserved.

引用

页码：2047 / 2051

页数：5

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