FLUOROMETRIC ASSAY OF ALPHA-METHYLENE CARBONYL-COMPOUNDS WITH N1-METHYLNICOTINAMIDE CHLORIDE

被引：31

作者：

NAKAMURA, H

TAMURA, Z

机构：

[1] Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Hongo 7-3-1, Bunkyo-ku

来源：

ANALYTICAL CHEMISTRY | 1978年 / 50卷 / 14期

关键词：

D O I：

10.1021/ac50036a027

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A spectrophotofluorometric method has been developed for the determination of α-methylene carbonyl compounds by using N1-methylnicotinamide chloride (NMN). When reacted at 0 °C for 10 min with NMN in the presence of alkali and heated at 92 °C for 3 min after the addition of formic acid solution, only compounds having an α-methylene carbonyl group (-CH2-CO-) gave intense violet to green fluorescence. By measuring the fluorescence, as little as 5 pmol of ace-tophenone, 1-indanone and α-tetralon could be determined. The relative standard deviations of 1.00% and 1.61% are observed for the analyses of 1 X 10ˉ 9 mol and 1 X 10ˉ10 mol of acetophenone, respectively. The mechanism associated with the reaction is also discussed. © 1978, American Chemical Society. All rights reserved.

引用

页码：2047 / 2051

页数：5

共 28 条

[21]

Johnson MJ, 1941, J BIOL CHEM, V137, P575

[22] FLUOROMETRIC ASSAY OF METHYL KETONES [J].

KRAMER, DN ;

TOLENTIN.LU ;

HACKLEY, EB .

ANALYTICAL CHEMISTRY, 1972, 44 (13) :2243-&

[23]

LEGAL E, 1883, JAHRESBER FORTSCHR C, P1648

[24]

LEVITAS N, 1947, J BIOL CHEM, V167, P169

[25]

NAKAMURA H, J CHROMATOGR

[26]

PESEZ M, 1969, ANN PHARM FR, V27, P161

[27] DETERMINATION OF CARBONYL COMPOUNDS BY 2-DIPHENYLACETYL-1,3-INDANDIONE-1-HYDRAZONE [J].

PIETRZYK, DJ ;

CHAN, EP .

ANALYTICAL CHEMISTRY, 1970, 42 (01) :37-&

[28]

ROBINSON J, 1947, J BIOL CHEM, V170, P653

← 1 2 3 →