A 6-HOUR MICRONEUTRALIZATION ASSAY FOR HUMAN CYTOMEGALOVIRUS ANTIBODY BY USING MONOCLONAL-ANTIBODIES

被引：20

作者：

GERNA, G

REVELLO, MG

PERCIVALLE, E

TORSELLINI, M

机构：

[1] Virus Laboratory, Institute of Infectious Diseases, University of Pavia

来源：

SERODIAGNOSIS AND IMMUNOTHERAPY IN INFECTIOUS DISEASE | 1990年 / 4卷 / 03期

关键词：

human cytomegalovirus antibody; microneutralization; monoclonal antibody;

D O I：

10.1016/0888-0786(90)90010-L

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Human cytomegalovirus (HCMV) was quantified at 6, 24, and 96 h post infection by counting the number of infected cells which were stained by the immunoperoxidase technique using monoclonal antibodies reactive to different HCMV non-structural and structural proteins. A linear dose-effect relationship was found at the three indicated times. Three rapid neutralization (Nt) assays (one for each time) were developed, using the same virus inoculum. Human sera from individuals with primary, reactivated or remote HCMV infection gave comparable Nt antibody titers with the three assays. The 6-hour rapid Nt assay appears highly suitable for determination of HCMV immune status and provides results the same day of blood collection. © 1990.

引用

页码：243 / 247

页数：5