NEUTROPHIL ACTIVATION BY MONOMERIC INTERLEUKIN-8

被引：277

作者：

RAJARATHNAM, K

SYKES, BD

KAY, CM

DEWALD, B

GEISER, T

BAGGIOLINI, M

CLARKLEWIS, I

机构：

[1] UNIV BRITISH COLUMBIA,DEPT BIOCHEM & MOLEC BIOL,BIOMED RES CTR,VANCOUVER V6T 1Z3,BC,CANADA

[2] UNIV ALBERTA,DEPT BIOCHEM,EDMONTON T6G 2S2,AB,CANADA

[3] UNIV BERN,THEODOR KOCHER INST,CH-3000 BERN,SWITZERLAND

[4] UNIV BRITISH COLUMBIA,PENCE,VANCOUVER V6T 1Z3,BC,CANADA

[5] UNIV ALBERTA,PROT ENGN NETWORK CTR EXCELLENCE,EDMONTON T6G 2S2,AB,CANADA

来源：

SCIENCE | 1994年 / 264卷 / 5155期

关键词：

D O I：

10.1126/science.8140420

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Interleukin-8 (IL-8), a pro-inflammatory protein, has been shown by nuclear magnetic resonance (NMR) and x-ray techniques to exist as a homodimer. An IL-8 analog was chemically synthesized, with the amide nitrogen of leucine-25 methylated to selectively block formation of hydrogen bonds between monomers.and thereby prevent dimerization. This analog was shown to be a monomer, as assessed by analytical ultracentrifugation and NMR. Nevertheless, it was equivalent to IL-8 in assays of neutrophil activation, which indicates that the monomer is a functional form of IL-8.

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页码：90 / 92

页数：3

相关论文

共 32 条

[1] INTERLEUKIN-8, A CHEMOTACTIC AND INFLAMMATORY CYTOKINE [J].

BAGGIOLINI, M ;

CLARKLEWIS, I .

FEBS LETTERS, 1992, 307 (01) :97-101

[2] CRYSTAL-STRUCTURE OF INTERLEUKIN-8 - SYMBIOSIS OF NMR AND CRYSTALLOGRAPHY [J].

BALDWIN, ET ;

WEBER, IT ;

STCHARLES, R ;

XUAN, JC ;

APPELLA, E ;

YAMADA, M ;

MATSUSHIMA, K ;

EDWARDS, BFP ;

CLORE, GM ;

GRONENBORN, AM ;

WLODAWER, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (02) :502-506

[3] CRYSTAL-STRUCTURE OF THE SOLUBLE HUMAN 55 KD TNF RECEPTOR-HUMAN TNF-BETA COMPLEX - IMPLICATIONS FOR TNF RECEPTOR ACTIVATION [J].

BANNER, DW ;

DARCY, A ;

JANES, W ;

GENTZ, R ;

SCHOENFELD, HJ ;

BROGER, C ;

LOETSCHER, H ;

LESSLAUER, W .

CELL, 1993, 73 (03) :431-445

[4] EQUILIBRIUM DISSOCIATION AND UNFOLDING OF THE ARC REPRESSOR DIMER [J].

BOWIE, JU ;

SAUER, RT .

BIOCHEMISTRY, 1989, 28 (18) :7139-7143

[5]

CLARKLEWIS I, 1991, J BIOL CHEM, V266, P23128

[6] PLATELET FACTOR-IV BINDS TO INTERLEUKIN-8 RECEPTORS AND ACTIVATES NEUTROPHILS WHEN ITS N-TERMINUS IS MODIFIED WITH GLU-LEU-ARG [J].

CLARKLEWIS, I ;

DEWALD, B ;

GEISER, T ;

MOSER, B ;

BAGGIOLINI, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (08) :3574-3577

[7] CHEMICAL SYNTHESIS, PURIFICATION, AND CHARACTERIZATION OF 2 INFLAMMATORY PROTEINS, NEUTROPHIL ACTIVATING PEPTIDE-1 (INTERLEUKIN-8) AND NEUTROPHIL ACTIVATING PEPTIDE-2 [J].

CLARKLEWIS, I ;

MOSER, B ;

WALZ, A ;

BAGGIOLINI, M ;

SCOTT, GJ ;

AEBERSOLD, R .

BIOCHEMISTRY, 1991, 30 (12) :3128-3135

[8] 3-DIMENSIONAL STRUCTURE OF INTERLEUKIN-8 IN SOLUTION [J].

CLORE, GM ;

APPELLA, E ;

YAMADA, M ;

MATSUSHIMA, K ;

GRONENBORN, AM .

BIOCHEMISTRY, 1990, 29 (07) :1689-1696

[9] HUMAN GROWTH-HORMONE AND EXTRACELLULAR DOMAIN OF ITS RECEPTOR - CRYSTAL-STRUCTURE OF THE COMPLEX [J].

DEVOS, AM ;

ULTSCH, M ;

KOSSIAKOFF, AA .

SCIENCE, 1992, 255 (5042) :306-312

[10] INTERLEUKIN-8 AND DEVELOPMENT OF ADULT RESPIRATORY-DISTRESS SYNDROME IN AT-RISK PATIENT GROUPS [J].

DONNELLY, SC ;

STRIETER, RM ;

KUNKEL, SL ;

WALZ, A ;

ROBERTSON, CR ;

CARTER, DC ;

GRANT, IS ;

POLLOK, AJ ;

HASLETT, C .

LANCET, 1993, 341 (8846) :643-647

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