CRYSTALLINE RIBULOSE 1,5-BISPHOSPHATE CARBOXYLASE-OXYGENASE FROM SPINACH

被引：12

作者：

JOHAL, S ^{[1
]}

BOURQUE, DP ^{[1
]}

机构：

[1] UNIV ARIZONA, DEPT BIOCHEM, TUCSON, AZ 85721 USA

来源：

SCIENCE | 1979年 / 204卷 / 4388期

关键词：

D O I：

10.1126/science.204.4388.75

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Spinach fraction I protein (ribulose 1,5-bisphosphate carboxylase- oxygenase, E.c. 4.1.1.39) was crystallized on both an analytical and a preparative scale by vapor diffusion with polyethylene glycol (molecular weight, 6000) used as the precipitant. The identity of the crystalline material with fraction I protein was shown by gel electrophoresis in the presence of sodium dodecyl sulfate and immunological properties. The carboxylase and oxygenase activities copurify during crystallization, and the crystalline enzyme lacks copper and iron. Copyright © 1979 AAAS.

引用

页码：75 / 77

页数：3

共 24 条

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