SEQUENCE VARIATION OF CYTOTOXIC T-CELL EPITOPES IN DIFFERENT ISOLATES OF EPSTEIN-BARR-VIRUS

被引：38

作者：

APOLLONI, A ^{[1
]}

MOSS, D ^{[1
]}

STUMM, R ^{[1
]}

BURROWS, S ^{[1
]}

SUHRBIER, A ^{[1
]}

MISKO, I ^{[1
]}

SCHMIDT, C ^{[1
]}

SCULLEY, T ^{[1
]}

机构：

[1] QUEENSLAND INST MED RES,300 HERSTON RD,BRISBANE,QLD 4029,AUSTRALIA

来源：

EUROPEAN JOURNAL OF IMMUNOLOGY | 1992年 / 22卷 / 01期

关键词：

D O I：

10.1002/eji.1830220127

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Previous results have identified two distinct cytotoxic T lymphocyte (CTL) epitopes encoded by Epstein-Barr virus (EBV), TETA (ORF BLRF3/BERF1 residues 329-353) and EENL (ORF BERF3/BERF4 residues 290-309). Measurement of the specificities of CTL clones (TETA-specific clone 13 and EENL-specific clone 7) directed against these epitopes indicated that the EENL epitope is conserved in all strains of EBV tested while the TETA epitope varied between individual virus strains. Sequencing of the DNA regions encoding these two CTL epitopes in different EBV isolates confirmed these interpretations and demonstrated that different TETA epitope sequences were encoded by B-type EBV strains and by the B95-8 isolate of EBV compared to the other A-type EBV strains. Titration of synthetic variants of the TETA epitope revealed that the epitope encoded by B95-8 was 15-fold less efficient as a T cell epitope than the sequence encoded by other A-type viral strains while the TETA variant encoded by the B-type strains displayed essentially no activity as a T cell epitope.

引用

页码：183 / 189

页数：7

共 23 条

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