STUDY OF PH-DEPENDENT STRUCTURAL TRANSITION IN TELOMERIC SEQUENCES BY 2-DIMENSIONAL GEL-ELECTROPHORESIS

被引：2

作者：

BELOTSERKOVSKII, BP ^{[1
]}

FRANKKAMENETSKII, MD ^{[1
]}

机构：

[1] RUSSIAN ACAD SCI,INST MOLEC GENET,MOSCOW 123182,RUSSIA

来源：

ELECTROPHORESIS | 1993年 / 14卷 / 04期

关键词：

D O I：

10.1002/elps.1150140147

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have applied the method of two-dimensional gel electrophoresis to study a pH-dependent structural transition in plasmid carrying the Tetrahymena telomeric sequence, (G4T2)n. A study of the two-dimensional patterns makes it possible to obtain energetic characteristics of the transition. We have obtained the nucleation energy of the structure. It proved to be two times lower than the nucleation energy of H-DNA determined before for a d(AG)n insert. The finding partly explains the formation of an eclectic structure in telomeric sequences. We also studied the effect of Zn++ ions on the two-dimensional patterns of gel electrophoresis. Our data show that Zn++ ions can significantly affect the transitions themselves. This finding leads to reinterpretation of numerous data on probing of structural transitions in DNA by the S1 nuclease.

引用

页码：266 / 270

页数：5

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