EFFECTS OF EXOGENOUS PROTEASE EFFECTORS ON BEEF TENDERNESS DEVELOPMENT AND MYOFIBRILLAR DEGRADATION AND SOLUBILITY

被引：81

作者：

UYTTERHAEGEN, L

CLAEYS, E

DEMEYER, D

机构：

[1] Department of Animal Production, University of Gent, Melle

来源：

JOURNAL OF ANIMAL SCIENCE | 1994年 / 72卷 / 05期

关键词：

BEEF; TENDERNESS; MUSCLE TISSUE; PROTEINASES; PROTEIN DEGRADATION; SDS-PAGE;

D O I：

10.2527/1994.7251209x

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

The effects of in situ postrigor injection (24 h postmortem) of exogenous aspartic, serine, and cysteine proteinase effecters into cylindrical beef longissimus samples on tenderness and myofibrillar protein degradation and integrity were studied. Injection of phenylmethanesulphonylfluoride (PMSF) and pepstatin did not influence shear force or protein degradation measured 8 d postmortem, confirming that neither serine nor aspartic proteinases affect tenderization. Injection of leupeptin, an epoxysuccinyl peptide (E-64), or N-acetyl-Leu-Leu-norleucinal (calpain inhibitor I) blocked tenderization completely, as observed by higher (P < .05) shear force values. A causal relationship between increased toughness and prevented action of the cysteine proteinases was suggested by a concomitant reduction of myofibrillar

引用

页码：1209 / 1223

页数：15

共 32 条

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