3-PHOTON INDUCED FLUORESCENCE OF 2,5-DIPHENYLOXAZOLE WITH A FEMTOSECOND TI-SAPPHIRE LASER

被引：54

作者：

GRYCZYNSKI, I

MALAK, H

LAKOWICZ, JR

机构：

[1] Center for Fluorescence Spectroscopy, Department of Biological Chemistry, University of Maryland School of Medicine, Baltimore, MD 21201

来源：

CHEMICAL PHYSICS LETTERS | 1995年 / 245卷 / 01期

关键词：

D O I：

10.1016/0009-2614(95)00958-7

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

We report emission spectra and time-resolved intensity and anisotropy decays resulting from three-photon excitation of fluorescence. The emission intensity of 2,5-diphenyloxazole (PPO) was found to depend on the third power of the excitation intensity at 870 nm. This wavelength is too long to excite PPO by a two-photon process. The emission spectrum, intensity decay, and rotational correlation times were found to be identical for one- (290 nm) and three-photon (870 nm) excitation. However, the time-zero anisotropy (r(0) = 0.61) observed from the time-resolved anisotropy decay, and observed in frozen solution, was larger than for one- or two-photon excitation. The possibility of three-photon excitation with a Ti:sapphire laser suggests wide ranging applications in biophysics and fluorescence microscopy.

引用

页码：30 / 35

页数：6

共 35 条

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