DIFFERENCES IN THE MACROMOLECULAR-COMPOSITION OF ZONA-PELLUCIDA ISOLATED FROM BOVINE OOCYTES, EMBRYOS AND A23187 PRETREATED OOCYTES

被引：11

作者：

BERCEGEAY, S ^{[1
]}

ALLAIRE, F ^{[1
]}

JEAN, M ^{[1
]}

LHERMITE, A ^{[1
]}

BRUYAS, JF ^{[1
]}

RENARD, N ^{[1
]}

TAINTURIER, D ^{[1
]}

BARRIERE, P ^{[1
]}

机构：

[1] ECOLE NATL VET NANTES,PATHOL REPROD LAB,F-44026 NANTES,FRANCE

来源：

REPRODUCTION NUTRITION DEVELOPMENT | 1993年 / 33卷 / 06期

关键词：

ZONA PELLUCIDA; BOVINE; SDS-PAGE; IEF; 2D-PAGE;

D O I：

10.1051/rnd:19930608

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The SDS-PAGE method was used to determine the composition of isolated bovine zona pellucida (ZP). This egg extracellular coat appears to be characterized by 3 major glycoproteins (ZP1, ZP2, ZP3), with apparent molecular weight ((MW) of 80-70 kD, 66-63 kD and 60 kD, respectively as revealed by I-dimensional SDS-PAGE. After P-dimensional SDS-PAGE, the zona pellucida electrophoretic pattern indicates a fourth glycoprotein, thus called ZP4. SDS-PAGE analysis of ZP isolated from oocytes and embryos after transit through the genital tract of A23187 pretreated oocytes allowed the description of modifications in glycoproteinic composition.

引用

页码：567 / 576

页数：10

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