CHARACTERIZATION OF CANINE PLATELET P-SELECTIN (CD-62) AND ITS UTILITY IN FLOW-CYTOMETRY PLATELET STUDIES

被引:27
作者
YEO, EL [1 ]
GEMMELL, CH [1 ]
SUTHERLAND, DR [1 ]
SEFTON, MV [1 ]
机构
[1] UNIV TORONTO, TORONTO HOSP, DEPT CHEM ENGN & APPL CHEM, TORONTO M5G 2C4, ON, CANADA
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1993年 / 105卷 / 3-4期
关键词
D O I
10.1016/0305-0491(93)90098-P
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
1. P-selectin (PADGEM, GMP140, or CD62) a member of lectin-like adhesive proteins is expressed on the surface of activated degranulated canine platelets and is the calcium-dependent receptor for leukocyte adhesion. 2. The electrophoretic mobility of P-selectin, by Western blot analysis and immunoprecipitation from radiolabeled membranes of canine and human platelets, was similar or identical and immunocytochemical studies localized P-selectin in internal vesicles similar to the alpha granule localization in human platelets. 3. Two antibodies to human P-selectin KC4.1 and AC1.2 crossreacted with canine platelets whose surface binding, in response to agonists thrombin, calcium ionophore (A23187), phorbol esters and ADP, was similar. 4. Anti-P-selectin antibodies in conjunction with crossreacting anti-GPIlb/IIIa antibodies (A2A9, 7E3, RUU-PL7F12) enables the analysis of activated platelets, platelet-derived microparticles and platelet-leukocyte interactions in canine models by flow cytometry.
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收藏
页码:625 / 636
页数:12
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