CHARACTERIZATION OF CANINE PLATELET P-SELECTIN (CD-62) AND ITS UTILITY IN FLOW-CYTOMETRY PLATELET STUDIES

被引：27

作者：

YEO, EL ^{[1
]}

GEMMELL, CH ^{[1
]}

SUTHERLAND, DR ^{[1
]}

SEFTON, MV ^{[1
]}

机构：

[1] UNIV TORONTO, TORONTO HOSP, DEPT CHEM ENGN & APPL CHEM, TORONTO M5G 2C4, ON, CANADA

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1993年 / 105卷 / 3-4期

关键词：

D O I：

10.1016/0305-0491(93)90098-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. P-selectin (PADGEM, GMP140, or CD62) a member of lectin-like adhesive proteins is expressed on the surface of activated degranulated canine platelets and is the calcium-dependent receptor for leukocyte adhesion. 2. The electrophoretic mobility of P-selectin, by Western blot analysis and immunoprecipitation from radiolabeled membranes of canine and human platelets, was similar or identical and immunocytochemical studies localized P-selectin in internal vesicles similar to the alpha granule localization in human platelets. 3. Two antibodies to human P-selectin KC4.1 and AC1.2 crossreacted with canine platelets whose surface binding, in response to agonists thrombin, calcium ionophore (A23187), phorbol esters and ADP, was similar. 4. Anti-P-selectin antibodies in conjunction with crossreacting anti-GPIlb/IIIa antibodies (A2A9, 7E3, RUU-PL7F12) enables the analysis of activated platelets, platelet-derived microparticles and platelet-leukocyte interactions in canine models by flow cytometry.

引用

页码：625 / 636

页数：12

共 49 条

[21] PADGEM PROTEIN - A RECEPTOR THAT MEDIATES THE INTERACTION OF ACTIVATED PLATELETS WITH NEUTROPHILS AND MONOCYTES [J].

LARSEN, E ;

CELI, A ;

GILBERT, GE ;

FURIE, BC ;

ERBAN, JK ;

BONFANTI, R ;

WAGNER, DD ;

FURIE, B .

CELL, 1989, 59 (02) :305-312

[22] PADGEM-DEPENDENT ADHESION OF PLATELETS TO MONOCYTES AND NEUTROPHILS IS MEDIATED BY A LINEAGE-SPECIFIC CARBOHYDRATE, LNF-III (CD15) [J].

LARSEN, E ;

PALABRICA, T ;

SAJER, S ;

GILBERT, GE ;

WAGNER, DD ;

FURIE, BC ;

FURIE, B .

CELL, 1990, 63 (03) :467-474

[23]

LEWIS JH, 1974, PLATELETS, P18

[24]

LIN SCH, 1984, J BIOL CHEM, V259, P9121

[25]

MCEVER RP, 1984, J BIOL CHEM, V259, P9799

[26]

MCEVER RP, 1987, BLOOD, V70, P355

[27]

METZELAAR MJ, 1991, VIRCHOWS ARCH B, V61, P269

[28]

MEYERS KM, 1986, PLATELET RESPONSES M, V1, pCH5

[29] INVIVO TC-99M S12 ANTIBODY IMAGING OF PLATELET ALPHA-GRANULES IN RABBIT ENDOTHELIAL NEOINTIMAL PROLIFERATION AFTER ANGIOPLASTY [J].

MILLER, DD ;

BOULET, AJ ;

TIO, FO ;

GARCIA, OJ ;

GUY, DM ;

MCEVER, RP ;

PALMAZ, JC ;

PAK, KY ;

NEBLOCK, DS ;

BERGER, HJ ;

DADDONA, PE .

CIRCULATION, 1991, 83 (01) :224-236

[30] P-SELECTIN (CD62) BINDS TO SUBPOPULATIONS OF HUMAN-MEMORY LYMPHOCYTES-T AND NATURAL-KILLER-CELLS [J].

MOORE, KL ;

THOMPSON, LF .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1992, 186 (01) :173-181

← 1 2 3 4 5 →