IDENTIFICATION AND STRUCTURAL CHARACTERIZATION OF A MANNOSE-6-PHOSPHATE CONTAINING OLIGOMANNOSIDIC N-GLYCAN FROM HUMAN ERYTHROPOIETIN SECRETED BY RECOMBINANT BHK-21-CELLS

被引：33

作者：

NIMTZ, M ^{[1
]}

WRAY, V ^{[1
]}

RUDIGER, A ^{[1
]}

CONRADT, HS ^{[1
]}

机构：

[1] GESELL BIOTECHNOL FORSCH MBH,DEPT CELL BIOL & GENET,D-38124 BRAUNSCHWEIG,GERMANY

来源：

FEBS LETTERS | 1995年 / 365卷 / 2-3期

关键词：

RECOMBINANT HUMAN ERYTHROPOIETIN; BABY HAMSTER KIDNEY CELLS (BHK-21); PHOSPORYLATED OLIGOMANNOSIDIC GLYCAN; MANNOSE-6-PHOSPHATE; 1- AND 2-D NMR SPECTROSCOPY; LYSOSOMAL RECOGNITION SIGNAL;

D O I：

10.1016/0014-5793(95)00473-M

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A sialidase resistant mono-charged N-glycan was isolated from glycosylation site I (Asn-24) of recombinant human erythropoietin expressed from baby hamster kidney (BHK-21) cells and constituted approximately 2-4% of the oligosaccharide material at this glycosylation site. Mass spectrometry and both 1- and 2-dimensional NMR techniques revealed a high mannose type structure (Man(6)) with a phospho-diesterbridged N-acetylglucosamine as follows: [GRAPHICS] and FK-506 binding proteins [Rahfeld et al, (1994) FEES Lett, 352, 180-184]. Should Ptf1p display PPIase activity, it would be the first characterized, eukaryotic member of this putative family, which is essential for growth.

引用

页码：203 / 208

页数：6

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