ALGAL PHOSPHOLIPIDS BY P-31 NMR - COMPARING ISOPROPANOL PRETREATMENT WITH SIMPLE CHLOROFORM/METHANOL EXTRACTION

被引：27

作者：

MENESES, P ^{[1
]}

NAVARRO, JN ^{[1
]}

GLONEK, T ^{[1
]}

机构：

[1] UNIV CATOLICA PUERTO RICO,DEPT BIOL,PONCE,PR

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY | 1993年 / 25卷 / 06期

关键词：

D O I：

10.1016/0020-711X(93)90246-B

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. A modified Folch procedure [potassium (ethylenedinitrilo)-tetraacetic acid at pH 6 substituted for KCl] is suitable for the extraction of marine algae. 2. The quantitative P-31 nuclear magnetic resonance phospholipid profiles of four marine algae, Gracilaria verrucosa, Bryothamnion triquetrum, Padina gymnospora, and Caulerpa sertularioides, were obtained from Folch and Nichols extractions of both fresh and dried algae, and essentially identical results were obtained using either extraction procedure. 3. Extracts of air-dried algae are statistically different when compared to extracts of living algae, suggesting that tissue handling is a critical factor in phospholipid extractions.

引用

页码：903 / 910

页数：8

共 34 条

[1] IMPROVED RESOLUTION OF P-31 NUCLEAR-MAGNETIC-RESONANCE SPECTRA OF PHOSPHOLIPIDS FROM BRAIN [J].