cAMP-dependent protein kinase in cerebral microvessels in aging and Alzheimer disease

被引：22

作者：

Cashman, RE ^{[1
]}

Grammas, P ^{[1
]}

机构：

[1] UNIV OKLAHOMA,HLTH SCI CTR,DEPT PATHOL,OKLAHOMA CITY,OK 73104

来源：

MOLECULAR AND CHEMICAL NEUROPATHOLOGY | 1995年 / 26卷 / 03期

关键词：

aging; Alzheimer disease; cerebral microvessels; blood-brain barrier; cAMP-dependent protein kinase; protein phosphorylation;

D O I：

10.1007/BF02815141

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The purpose of this study was to compare the effects of aging and Alzheimer disease (AD) on the important intracellular signaling enzyme cAMP-dependent protein kinase A (PKA) in cerebral microvessels. PKA activity and levels were measured in microvessels isolated from the brains of adult and aged rodents as well as from the cerebral cortices of AD and elderly control patients. The results showed that cerebral microvessels from aged rats have significantly (p < 0.01) higher PKA activity and levels when compared to cerebral microvessels from adult rats. In contrast, no significant difference was found between PKA activity or levels in cerebral microvessels from AD patients when compared to controls. These results indicate that in cerebral microvessels both PKA activity and levels increase with age but are unaffected by AD. The data suggest that protein phosphorylation in brain microvessels may be affected differentially by aging and dementia.

引用

页码：247 / 258

页数：12

共 37 条

[1] PROTEIN-PHOSPHORYLATION - CHANGES WITH AGE AND AGE-RELATED DISEASES [J].

ARMBRECHT, HJ ;

NEMANI, RK ;

WONGSURAWAT, N .

JOURNAL OF THE AMERICAN GERIATRICS SOCIETY, 1993, 41 (08) :873-879

[2]

Ball M J, 1988, Alzheimer Dis Assoc Disord, V2, P29, DOI 10.1097/00002093-198802010-00004

[3] MORPHOMETRIC COMPARISON OF HIPPOCAMPAL MICROVASCULATURE IN AGING AND DEMENTED PEOPLE - DIAMETERS AND DENSITIES [J].

BELL, MA ;

BALL, MJ .

ACTA NEUROPATHOLOGICA, 1981, 53 (04) :299-318

[4]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[5] SELECTIVE ALTERATIONS OF HIGH-AFFINITY [H-3] FORSKOLIN BINDING-SITES IN ALZHEIMERS-DISEASE - A QUANTITATIVE AUTORADIOGRAPHIC STUDY [J].

DEWAR, D ;

HORSBURGH, K ;

GRAHAM, DI ;

BROOKS, DN ;

MCCULLOCH, J .

BRAIN RESEARCH, 1990, 511 (02) :241-248

[6]

DIGLIO CA, 1982, LAB INVEST, V46, P554

[7] EFFECT OF AGE ON BRAIN CORTICAL PROTEIN KINASE-C AND ITS MEDIATION OF 5-HYDROXYTRYPTAMINE RELEASE [J].

FRIEDMAN, E ;

WANG, HY .

JOURNAL OF NEUROCHEMISTRY, 1989, 52 (01) :187-192

[8] A PROTEIN BINDING ASSAY FOR ADENOSINE 3'-5'-CYCLIC MONOPHOSPHATE [J].

GILMAN, AG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1970, 67 (01) :305-&

[9] REDUCED CAMP-DEPENDENT PHOSPHORYLATION IN STRIATUM AND NUCLEUS ACCUMBENS OF AGED RATS - EVIDENCE OF AN ALTERED FUNCTIONING OF D1 DOPAMINOCEPTIVE NEURONS [J].

GOVONI, S ;

RIUS, RA ;

BATTAINI, F ;

TRABUCCHI, M .

JOURNALS OF GERONTOLOGY, 1988, 43 (04) :B93-B97

[10] CEREBRAL MICROVESSELS IN ALZHEIMERS HAVE REDUCED PROTEIN-KINASE-C ACTIVITY [J].

GRAMMAS, P ;

MOORE, P ;

BOTCHLET, T ;

HANSONPAINTON, O ;

COOPER, DR ;

BALL, MJ ;

ROHER, A .

NEUROBIOLOGY OF AGING, 1995, 16 (04) :563-569

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