YERSINIA-TRIGGERED REACTIVE ARTHRITIS - USE OF POLYMERASE CHAIN-REACTION AND IMMUNOCYTOCHEMICAL STAINING IN THE DETECTION OF BACTERIAL COMPONENTS FROM SYNOVIAL SPECIMENS

被引：104

作者：

NIKKARI, S

MERILAHTIPALO, R

SAARIO, R

SODERSTROM, KO

GRANFORS, K

SKURNIK, M

TOIVANEN, P

机构：

[1] UNIV TURKU,DEPT MED,SF-20520 TURKU 52,FINLAND

[2] UNIV TURKU,DEPT PATHOL,SF-20520 TURKU 52,FINLAND

来源：

ARTHRITIS AND RHEUMATISM | 1992年 / 35卷 / 06期

关键词：

D O I：

10.1002/art.1780350613

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Objective. To investigate whether microbial DNA is present in synovial specimens from patients with Yersinia-triggered reactive arthritis. Methods. Synovial specimens from 13 patients with Yersinia enterocolitica O:3-triggered reactive arthritis and from 16 control patients were studied using polymerase chain reaction and immunocytochemical staining techniques. Results. Yersinia chromosomal DNA was not found in any of the synovial specimens from Yersinia-triggered arthritis patients or controls, whereas with immunocytochemical techniques, Yersinia antigens were observed in synovial specimens from all of the patients with Yersinia-triggered reactive arthritis. Conclusion. Only stable bacterial degradation products, not whole bacteria, are present at the site of inflammation in Yersinia-triggered reactive arthritis.

引用

页码：682 / 687

页数：6

共 15 条

[11] INTERVENING SEQUENCES (IVSS) IN THE 23S RIBOSOMAL-RNA GENES OF PATHOGENIC YERSINIA-ENTEROCOLITICA STRAINS - THE IVSS IN Y-ENTEROCOLITICA AND SALMONELLA-TYPHIMURIUM HAVE A COMMON ORIGIN [J].