THE RETARDED ELECTROPHORETIC MIGRATION OF P56LCK INDUCED BY VANADATE IN LYMPHOMA-CELLS CORRELATES WITH MODIFIED KINASE-ACTIVITY

被引：5

作者：

DANIELIAN, S ^{[1
]}

FAGARD, R ^{[1
]}

BOULET, I ^{[1
]}

FISCHER, S ^{[1
]}

机构：

[1] INSERM,U332,24 RUE FAUBOURG ST JACQUES,F-75014 PARIS,FRANCE

来源：

BIOCHIMIE | 1990年 / 72卷 / 05期

关键词：

lymphocyte; p56[!sup]lck[!/sup; phosphorylation; tyrosine protein kinase; vanadate;

D O I：

10.1016/0300-9084(90)90026-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

p56lck is a src related lymphocyte specific tyrpsine protein kinase which undergoes specific changes during T-cell activation, particularly the appearance of slow migrating forms. To analyze these forms, LSTRA cells were treated with vanadate. This resulted in increased phosphorylation of p56lck with the appearance of slow migrating forms. Renaturation of the p56lck bands after gel migration showed that vandate mostly increased the activity of the lower band of p56lck. The upper bands had a reduced specific activity. In addition, the upper bands from vanadate treated cells displayed additional phosphorylated sites. © 1990.

引用

页码：315 / 322

页数：8

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