THE RETARDED ELECTROPHORETIC MIGRATION OF P56LCK INDUCED BY VANADATE IN LYMPHOMA-CELLS CORRELATES WITH MODIFIED KINASE-ACTIVITY

被引：5

作者：

DANIELIAN, S ^{[1
]}

FAGARD, R ^{[1
]}

BOULET, I ^{[1
]}

FISCHER, S ^{[1
]}

机构：

[1] INSERM,U332,24 RUE FAUBOURG ST JACQUES,F-75014 PARIS,FRANCE

来源：

BIOCHIMIE | 1990年 / 72卷 / 05期

关键词：

lymphocyte; p56[!sup]lck[!/sup; phosphorylation; tyrosine protein kinase; vanadate;

D O I：

10.1016/0300-9084(90)90026-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

p56lck is a src related lymphocyte specific tyrpsine protein kinase which undergoes specific changes during T-cell activation, particularly the appearance of slow migrating forms. To analyze these forms, LSTRA cells were treated with vanadate. This resulted in increased phosphorylation of p56lck with the appearance of slow migrating forms. Renaturation of the p56lck bands after gel migration showed that vandate mostly increased the activity of the lower band of p56lck. The upper bands had a reduced specific activity. In addition, the upper bands from vanadate treated cells displayed additional phosphorylated sites. © 1990.

引用

页码：315 / 322

页数：8

共 53 条

[11]

CASNELLIE JE, 1983, J BIOL CHEM, V258, P738

[12]

CLEELAND D, 1978, J BIOL CHEM, V257, P1102

[13]

CZECH MP, 1988, J BIOL CHEM, V263, P11017

[14] THE LYMPHOCYTE-SPECIFIC PROTEIN TYROSINE KINASE P56LCK IS HYPERPHOSPHORYLATED ON SERINE AND TYROSINE RESIDUES WITHIN MINUTES AFTER ACTIVATION VIA T-CELL RECEPTOR OR CD2 [J].

DANIELIAN, S ;

FAGARD, R ;

ALCOVER, A ;

ACUTO, O ;

FISCHER, S .

EUROPEAN JOURNAL OF IMMUNOLOGY, 1989, 19 (12) :2183-2189

[15]

DUBYAK GR, 1980, J BIOL CHEM, V255, P5306

[16]

EARP HS, 1983, FEBS LETT, V161, P180

[17] DETECTION OF TYROSINE-SPECIFIC PROTEIN-KINASES WITH GASTRIN AS EXOGENOUS SUBSTRATE [J].

FAGARD, R ;

GACON, G ;

BOISSEL, JP ;

REIBEL, L ;

PIAU, JP ;

FISCHER, S ;

SCHAPIRA, G ;

ACCARY, JP .

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 1985, 10 (5-6) :307-314

[18] THE AMINO TERMINAL REGION OF THE P56-LCK FROM LSTRA EXERTS NEGATIVE MODULATION ON THE TYROSINE KINASE-ACTIVITY [J].

FISCHER, S ;

FAGARD, R ;

BOULET, I ;

GESQUIERE, JC .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1987, 143 (03) :819-826

[19] STIMULATION OF TYROSINE PHOSPHORYLATION IN LECTIN TREATED HUMAN-LYMPHOCYTES [J].

FISCHER, S ;

FAGARD, R ;

GACON, G ;

GENETET, N ;

PIAU, JP ;

BLAINEAU, C .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1984, 124 (03) :682-689

[20] IDENTIFICATION OF A 58,000 DALTONS PHOSPHOPROTEIN WITH TYROSINE PROTEIN-KINASE ACTIVITY IN A MURINE LYMPHOMA CELL-LINE [J].

GACON, G ;

FAGARD, R ;

BOISSEL, JP ;

FISCHER, S ;

REIBEL, L ;

PIAU, JP ;

SCHAPIRA, G ;

COMOGLIO, PM .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1984, 122 (02) :563-570

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