THE LAST 7 TRANSMEMBRANE AND CARBOXY-TERMINAL CYTOPLASMIC DOMAINS OF EPSTEIN-BARR-VIRUS LATENT MEMBRANE-PROTEIN 2 (LMP2) ARE DISPENSABLE FOR LYMPHOCYTE INFECTION AND GROWTH TRANSFORMATION INVITRO

被引:98
作者
LONGNECKER, R [1 ]
MILLER, CL [1 ]
MIAO, XQ [1 ]
TOMKINSON, B [1 ]
KIEFF, E [1 ]
机构
[1] HARVARD UNIV,SCH MED,DEPT MOLEC GENET,BOSTON,MA 02115
关键词
D O I
10.1128/JVI.67.4.2006-2013.1993
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Specifically mutated Epstein-Barr virus (EBV) recombinants which truncate latent membrane protein 2A (LMP2A) and LMP2B after 260 of 497 amino acids and after 141 of 378 amino acids, respectively, were constructed. Despite truncation before the last seven transmembrane domains and the carboxy terminus, the mutant recombinants were not altered in initiation of primary B-lymphocyte infection or growth transformation, in expression of nuclear protein 1 or 2 or LMP1, or in induction of lytic EBV replication. Cells transformed by mutant virus recombinants were not different from wild-type virus transformants in initial or long-term outgrowth, sensitivity to limiting cell dilution, serum requirement, or clonogenic growth in soft agar. Together with similar analyses of a mutation stopping translation of the LMP2A amino-terminal cytoplasmic domain, these results indicate that LMP2 is not required for primary B-lymphocyte infection in vitro.
引用
收藏
页码:2006 / 2013
页数:8
相关论文
共 43 条
[31]   2 RELATED EPSTEIN-BARR VIRUS MEMBRANE-PROTEINS ARE ENCODED BY SEPARATE GENES [J].
SAMPLE, J ;
LIEBOWITZ, D ;
KIEFF, E .
JOURNAL OF VIROLOGY, 1989, 63 (02) :933-937
[32]   MALIGNANT LYMPHOMA IN COTTONTOP MARMOSETS AFTER INOCULATION WITH EPSTEIN-BARR VIRUS [J].
SHOPE, T ;
DECHAIRO, D ;
MILLER, G .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1973, 70 (09) :2487-2491
[33]  
STAAL SP, 1989, AM J CLIN PATHOL, V91, P1
[34]   THE NATURE OF HODGKIN AND REED-STERNBERG CELLS, THEIR ASSOCIATION WITH EBV, AND THEIR RELATIONSHIP TO ANAPLASTIC LARGE-CELL LYMPHOMA [J].
STEIN, H ;
HERBST, H ;
ANAGNOSTOPOULOS, I ;
NIEDOBITEK, G ;
DALLENBACH, F ;
KRATZSCH, HC .
ANNALS OF ONCOLOGY, 1991, 2 :33-38
[35]   CLONAL TRANSFORMATION OF ADULT HUMAN LEUKOCYTES BY EPSTEIN-BARR VIRUS [J].
SUGDEN, B ;
MARK, W .
JOURNAL OF VIROLOGY, 1977, 23 (03) :503-508
[36]  
SWQAMINATHAN S, 1991, P NATL ACAD SCI USA, V88, P1546
[37]   USE OF 2ND-SITE HOMOLOGOUS RECOMBINATION TO DEMONSTRATE THAT EPSTEIN-BARR-VIRUS NUCLEAR PROTEIN-3B IS NOT IMPORTANT FOR LYMPHOCYTE INFECTION OR GROWTH TRANSFORMATION INVITRO [J].
TOMKINSON, B ;
KIEFF, E .
JOURNAL OF VIROLOGY, 1992, 66 (05) :2893-2903
[38]   2ND-SITE HOMOLOGOUS RECOMBINATION IN EPSTEIN-BARR-VIRUS - INSERTION OF TYPE-1 EBNA-3 GENES IN PLACE OF TYPE-2 HAS NO EFFECT ON INVITRO INFECTION [J].
TOMKINSON, B ;
KIEFF, E .
JOURNAL OF VIROLOGY, 1992, 66 (02) :780-789
[39]   HU-PBL-SCID MICE - A MODEL FOR HUMAN IMMUNE FUNCTION, AIDS, AND LYMPHOMAGENESIS [J].
TORBETT, BE ;
PICCHIO, G ;
MOSIER, DE .
IMMUNOLOGICAL REVIEWS, 1991, 124 :139-164
[40]   EPSTEIN-BARR-VIRUS (EBV) RECOMBINANTS - USE OF POSITIVE SELECTION MARKERS TO RESCUE MUTANTS IN EBV-NEGATIVE B-LYMPHOMA CELLS [J].
WANG, F ;
MARCHINI, A ;
KIEFF, E .
JOURNAL OF VIROLOGY, 1991, 65 (04) :1701-1709