ELECTRON-PARAMAGNETIC RESONANCE SPECTROMETER FOR 3-DIMENSIONAL INVIVO IMAGING AT VERY LOW-FREQUENCY

被引:44
作者
ALECCI, M
DELLAPENNA, S
SOTGUI, A
TESTA, L
VANNUCCI, I
机构
[1] Dipartimento di Scienze e Tecnologie Biomediche, Università dell'Aquila
关键词
D O I
10.1063/1.1143723
中图分类号
TH7 [仪器、仪表];
学科分类号
0804 ; 080401 ; 081102 ;
摘要
The paper describes an electron paramagnetic resonance apparatus for spectroscopy and imaging at very low frequency (283 MHz). The bridge operates in a reflection cavity homodyne configuration and can be used on a very broad frequency range. The sample cavity is a one loop-two gap resonator and accepts samples up to 5 cm in diameter and 10 cm long. These sample dimensions make the apparatus suitable for observing living samples such as rats. For the main field and gradients the apparatus uses a newly designed multipolar magnet that can provide the main field and gradients for 2D image reconstruction. The third gradient is obtained by coils mounted in the bore of the magnet. Tests on peroxilamine disulfonate samples have shown micromolar sensitivity. The minimum spatial resolution is limited by the sample linewidth and by the signal/noise. In living samples the toxicity of nitroxide radicals restricts the maximum label concentrations to values of the order of 10(-4) M, and the resolution seems to be limited by sensitivity to fractions of cm3.
引用
收藏
页码:4263 / 4270
页数:8
相关论文
共 35 条
[1]   3-DIMENSIONAL INVIVO ESR IMAGING IN RATS [J].
ALECCI, M ;
COLACICCHI, S ;
INDOVINA, PL ;
MOMO, F ;
PAVONE, P ;
SOTGIU, A .
MAGNETIC RESONANCE IMAGING, 1990, 8 (01) :59-63
[2]   LUMPED PARAMETERS DESCRIPTION OF RF LOSSES IN ESR EXPERIMENTS ON ELECTRICALLY CONDUCTING SAMPLES [J].
ALECCI, M ;
GUALTIERI, G ;
SOTGIU, A .
JOURNAL OF PHYSICS E-SCIENTIFIC INSTRUMENTS, 1989, 22 (06) :354-359
[3]   MULTIPOLAR MAGNET FOR LOW-FREQUENCY ESR IMAGING [J].
ALECCI, M ;
GUALTIERI, G ;
SOTGIU, A ;
TESTA, L ;
VAROLI, V .
MEASUREMENT SCIENCE AND TECHNOLOGY, 1991, 2 (01) :32-37
[4]  
BAKER J E, 1990, Free Radical Biology and Medicine, V9, P93, DOI 10.1016/0891-5849(90)90500-I
[5]  
BASIC G, 1989, MAGNET RESON MED, V10, P266
[6]   INVIVO PHARMACOKINETICS BY ELECTRON MAGNETIC-RESONANCE SPECTROSCOPY [J].
BERLINER, LJ ;
WAN, XM .
MAGNETIC RESONANCE IN MEDICINE, 1989, 9 (03) :430-434
[7]   FEASIBILITY STUDY OF IMAGING A LIVING MURINE TUMOR BY ELECTRON-PARAMAGNETIC RESONANCE [J].
BERLINER, LJ ;
FUJII, H ;
WAN, XM ;
LUKIEWICZ, SJ .
MAGNETIC RESONANCE IN MEDICINE, 1987, 4 (04) :380-384
[8]   RF MAGNETIC-FIELD PENETRATION, PHASE-SHIFT AND POWER DISSIPATION IN BIOLOGICAL TISSUE - IMPLICATIONS FOR NMR IMAGING [J].
BOTTOMLEY, PA ;
ANDREW, ER .
PHYSICS IN MEDICINE AND BIOLOGY, 1978, 23 (04) :630-643
[9]   TECHNIQUE FOR MEASUREMENT OF TISSUE IMPEDANCE FROM 1 TO 100 MHZ USING A VECTOR IMPEDANCE METER [J].
BOTTOMLEY, PA .
JOURNAL OF PHYSICS E-SCIENTIFIC INSTRUMENTS, 1978, 11 (05) :413-414
[10]  
BRIVATI JA, 1991, J MAGN RESON, V92