CLONING OF COLIPHAGE-T4 GENE PSET AND HIGH-LEVEL SYNTHESIS OF POLYNUCLEOTIDE KINASE IN ESCHERICHIA-COLI

被引：4

作者：

CAMPOS, M

ORTEGA, M

PADRON, G

ESTRADA, MP

DELAFUENTE, J

HERRERA, L

机构：

[1] CTR INVEST GENET BIOL,CTR GENET ENGN & BIOTECHNOL,ANALYT UNIT,HAVANA,CUBA

[2] CTR INVEST GENET BIOL,ENZIBIOT,HAVANA,CUBA

来源：

GENE | 1991年 / 101卷 / 01期

关键词：

RECOMBINANT DNA; GENE CLONING; EXPRESSION PLASMID DERIVATIVES; POLYMERASE CHAIN REACTION; TRP PROMOTER;

D O I：

10.1016/0378-1119(91)90234-3

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The gene, pseT, of coliphage T4 which encodes polynucleotide kinase (PNK) was cloned directly into an expression plasmid using the polymerase chain reaction. When placed under the control of the trp promoter, the pseT gene can be maintained stably in Escherichia coli and yields high levels of the enzyme upon induction. The system described facilitates purification and provides very high yields of PNK.

引用

收藏

页码：127 / 131

页数：5

相关论文

共 11 条

[1]

BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513

[2] 3'-PHOSPHATASE ACTIVITY IN T4 POLYNUCLEOTIDE KINASE [J].

CAMERON, V ;

UHLENBECK, OC .

BIOCHEMISTRY, 1977, 16 (23) :5120-5126

[3] SUPERCOIL SEQUENCING - A FAST AND SIMPLE METHOD FOR SEQUENCING PLASMID DNA [J].

CHEN, EY ;

SEEBURG, PH .

DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY, 1985, 4 (02) :165-170

[4] PLASMID VECTORS CONTAINING THE TRYPTOPHAN OPERON PROMOTER SUITABLE FOR EFFICIENT REGULATED EXPRESSION OF FOREIGN GENES [J].

HALLEWELL, RA ;

EMTAGE, S .

GENE, 1980, 9 (1-2) :27-47

[5] CHAIN-LENGTH DETERMINATION OF SMALL DOUBLE-STRANDED AND SINGLE-STRANDED-DNA MOLECULES BY POLYACRYLAMIDE-GEL ELECTROPHORESIS [J].

MANIATIS, T ;

JEFFREY, A ;

VANDESANDE, H .

BIOCHEMISTRY, 1975, 14 (17) :3787-3794

[6] T4 POLYNUCLEOTIDE KINASE - CLONING OF THE GENE (PSET) AND AMPLIFICATION OF ITS PRODUCT [J].

MIDGLEY, CA ;

MURRAY, NE .

EMBO JOURNAL, 1985, 4 (10) :2695-2703

[7] STUDIES ON POLYNUCLEOTIDES .123. PHYSICAL CHARACTERIZATION AND SIMULTANEOUS PURIFICATION OF BACTERIOPHAGE-T4 INDUCED POLYNUCLEOTIDE KINASE, POLYNUCLEOTIDE LIGASE, AND DEOXYRIBONUCLEIC ACID POLYMERASE [J].

PANET, A ;

VANDESAN.JH ;

LOEWEN, PC ;

KHORANA, HG ;

RAAE, AJ ;

LILLEHAUG, JR ;

KLEPPE, K .

BIOCHEMISTRY, 1973, 12 (25) :5045-5050

[8]

PLATT T, 1980, OPERON, P263

[9] PHOSPHORYLATION OF NUCLEIC ACID BY AN ENZYME FROM T4 BACTERIOPHAGE-INFECTED ESCHERICHIA COLI [J].

RICHARDSON, CC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1965, 54 (01) :158-+

[10] ENZYMATIC AMPLIFICATION OF BETA-GLOBIN GENOMIC SEQUENCES AND RESTRICTION SITE ANALYSIS FOR DIAGNOSIS OF SICKLE-CELL ANEMIA [J].

SAIKI, RK ;

SCHARF, S ;

FALOONA, F ;

MULLIS, KB ;

HORN, GT ;

ERLICH, HA ;

ARNHEIM, N .

SCIENCE, 1985, 230 (4732) :1350-1354