SOMA-SPECIFIC EXPRESSION AND CLONING OF PSI, A NEGATIVE REGULATOR OF P-ELEMENT PRE-MESSENGER-RNA SPLICING

被引:115
作者
SIEBEL, CW
ADMON, A
RIO, DC
机构
[1] UNIV CALIF BERKELEY,DEPT MOLEC & CELL BIOL,DIV GENET,BERKELEY,CA 94720
[2] UNIV CALIF BERKELEY,DIV BIOCHEM & MOLEC BIOL,BERKELEY,CA 94720
[3] UNIV CALIF BERKELEY,HOWARD HUGHES MED INST,BERKELEY,CA 94720
关键词
DROSOPHILA; SPLICING; P ELEMENT; PSI; RNA-BINDING PROTEIN; TISSUE SPECIFICITY;
D O I
10.1101/gad.9.3.269
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
PSI is an RNA-binding protein involved in repressing splicing of the P element third intron in Drosophila somatic cell extracts. PSI produced in bacteria restores splicing inhibition to an extract relieved of inhibitory activity, indicating that PSI plays a direct role in somatic inhibition. Sequence analysis of cDNAs encoding PSI reveals three KH RNA-binding domains, a conserved motif also found in the yeast splicing regulator MER1. Notably, PSI is expressed highly in somatic embryonic nuclei but is undetectable in germ-line cells. In contrast, hrp48, another protein implicated in somatic inhibition, is found in the nucleus and cytoplasm of both tissues. The splicing inhibitory properties and soma-specific expression of PSI may be sufficient to explain the germ-line-specific transposition of P elements.
引用
收藏
页码:269 / 283
页数:15
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