EXPRESSION PATTERN OF URICASE-II GENE DURING ROOT-NODULE DEVELOPMENT IN PHASEOLUS-VULGARIS

被引：14

作者：

PAPADOPOULOU, K

ROUSSIS, A

KUIN, H

KATINAKIS, P

机构：

[1] AGR UNIV ATHENS, DEPT AGR BIOL & BIOTECHNOL, GR-11855 ATHENS, GREECE

[2] AGR UNIV WAGENINGEN, DEPT MOLEC BIOL, 6703 HA WAGENINGEN, NETHERLANDS

来源：

EXPERIENTIA | 1995年 / 51卷 / 01期

关键词：

URICASE II; PHASEOLUS; NODULIN; NITROGEN FIXATION;

D O I：

10.1007/BF01964927

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A Phaseolus vulgaris uricase II cDNA clone has been isolated and sequenced. Comparison on the nucleotide level between this clone and the soybean uricase II clone revealed 88.8% identity. The in situ hybridization technique was used to follow the expression pattern in developing root nodules of Phaseolus vulgaris. The uricase II transcripts were localized only in the uninfected cells of the central tissue and mainly in the periphery of the cell. Uricase II mRNA is first detected in nodules 12 days after infection. A maximum level of transcripts is reached in 21-day-old nodules, followed by a considerable reduction in 28-day-old nodules.

引用

页码：90 / 94

页数：5

共 37 条

[21] UNINFECTED CELLS OF SOYBEAN ROOT-NODULES - ULTRASTRUCTURE SUGGESTS KEY ROLE IN UREIDE PRODUCTION [J].