DETECTION OF HDV-RNA BY PCR IN SERUM OF PATIENTS WITH CHRONIC HDV INFECTION

被引：28

作者：

MADEJON, A

CASTILLO, I

BARTOLOME, J

MELERO, M

CAMPILLO, ML

PORRES, JC

MORENO, A

CARRENO, V

机构：

[1] FDN JIMENEZ DIAZ,DEPT GASTROENTEROL,AVENIDA REYES CATOLICOS 2,E-28040 MADRID,SPAIN

[2] HOSP RAMON & CAJAL,DEPT PATHOL,MADRID,SPAIN

来源：

JOURNAL OF HEPATOLOGY | 1990年 / 11卷 / 03期

关键词：

D O I：

10.1016/0168-8278(90)90225-G

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

In this paper, we studied the usefulness of polymer ase chain reaction (PCR) in HDV-RNA detection. Using serial dilutions of serum samples of known concentrations of HDV-RNA, PCR was 10 000-times more sensitive than slot-blot hybridization. PCR was used for the detection of HDV-RNA in 33 serum samples negative to HDV-RNA by conventional slot-blot hybridization. HDV-RNA was detected in 18 33 (54%) of the samples included in this study using PCR. When positivity to a viral genome was related to other viral replication markers, it was found that among the 18 patients positive to the viral genome, 13 (72%) had hepatitis delta antigen in the liver, and five (28%) were negative. In conclusion, HDV-RNA detection by gene amplification is 10 000-times more sensitive than slot-blot hybridization, and allows the detection of viral replication in patients without ether viral replication markers. © 1990.

引用

页码：381 / 384

页数：4