SENSITIVITY OF ACTINOBACILLUS-ACTINOMYCETEMCOMITANS AND HAEMOPHILUS-APHROPHILUS TO OXIDATIVE KILLING

被引：13

作者：

DONGARI, AI ^{[1
]}

MIYASAKI, KT ^{[1
]}

机构：

[1] UNIV CALIF LOS ANGELES,CTR HLTH SCI,SCH DENT,ORAL BIOL SECT,CHS 63-050,LOS ANGELES,CA 90024

来源：

ORAL MICROBIOLOGY AND IMMUNOLOGY | 1991年 / 6卷 / 06期

关键词：

BACTERICIDAL; XANTHINE XANTHINE OXIDASE; HYDROGEN PEROXIDE; SUPEROXIDE ANION; OXIDATIVE KILLING; ACTINOBACILLUS-ACTINOMYCETEMCOMITANS; HAEMOPHILUS-APHROPHILUS;

D O I：

10.1111/j.1399-302X.1991.tb00508.x

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

We examined the killing of Actinobacillus actinomycetemcomitans and Haemophilus aphrophilus by oxygen metabolites generated by the xanthine-xanthine oxidase (X-XO) system. This system generates a mixture of oxidants, including superoxide radical, hydrogen peroxide, hydroxyl radical, and possibly singlet oxygen. Differential sensitivity to the X-XO system was observed among strains of A. actinomycetemcomitans; notably, 2 catalase-deficient strains and 2 strains representative of serotypes b and c were the most susceptible. H. aphrophilus was not sensitive. The amount of oxidants produced by the X-XO system more closely correlated with killing than the ratio of oxidant production. Cytochrome c, superoxide dismutase, catalase, dimethyl sulfoxide, and desferrioxamine were used to determine the role of superoxide radical, hydrogen peroxide and hydroxyl radical in the bactericidal process. Hydrogen peroxide was the major bactericidal agent against A. actinomycetemcomitans. Superoxide anion participated in killing of A. actinomycetemcomitans to varying but lesser degrees. The intracellular generation of hydroxyl radical was implicated in the killing of several strains. We conclude that (i) strains of A. actinomycetemcomitans are differentially sensitive to the bactericidal effects of the X-XO system and (ii) of the oxidants produced by the X-XO system, hydrogen peroxide is the most bactericidal against A. actinomycetemcomitans.

引用

页码：363 / 372

页数：10

共 43 条

[31] Pick E, Mizel D, Rapid microassays for the measurement of superoxide and hydrogen peroxide production by macro‐phages in culture using an automatic enzyme immunoassay reader, J Immunol Methods, 46, pp. 211-226, (1981)
[32] Repine JE, Fox RB, Berger EM, Hydrogen peroxide kills Staphylococcus aureus by reacting with staphylococcal iron to form hydroxyl radical, J Biol Chem, 256, pp. 7094-7096, (1981)
[33] Rest RF, Spitznagel JK, MPO‐Cr‐H2O2 bactericidal system: effect on bacterial membrane structure and growth conditions, Infect Immun, 19, pp. 1110-1112, (1978)
[34] Roos D, The metabolic response to phagocytosis, Handbook of inflammation, 2, pp. 337-385, (1980)
[35] Roos M, Roos D, Differences in oxygen metabolism of phagocytosing monocyles and neutrophils, J Clin Invest, 61, pp. 480-488, (1978)
[36] Segal AW, Cytochrome b‐245 and its involvement in the molecular pathology of chronic granulomatous disease, Hematol Oncol Clin North Am, 2, pp. 213-223, (1988)
[37] Slots J, Reynolds HS, Genco RJ, Actinobacillus actinomycetemcomitans in human periodontal disease: a cross sectional microbiological investigation, Infect Immun, 29, pp. 1013-1020, (1980)
[38] Slots J, Salient biochemical characters of Actinobacillus actinomycetemcomitans, Arch Microbiol, 13, pp. 60-67, (1982)
[39] Taichman NS, Tsai CC, Shenker BJ, Boehringer HR, Neutrophil interactions with oral bacteria as a pathogenic mechanism in periodontal diseases, Adv In-flam Res, 8, pp. 113-142, (1984)
[40] van Steveninck J, van der Zee J, Dubbelman TMAR, Site‐specific and bulk‐phase generation of hydroxyl radicals in the presence of cupric ions and thiol compounds, Biochem J, 232, pp. 309-311, (1985)

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