6-METHYLSALICYLIC ACID DECARBOXYLASE FROM PENICILLIUM PATULUM

被引：29

作者：

LIGHT, RJ

机构：

[1] Department of Chemistry, Florida State University, Tallahassee

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1969年 / 191卷 / 02期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/0005-2744(69)90262-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. 1. A crude extract catalyzing the decarboxylation of 6-methylsalicyclic acid has been isolated from Penicillium patulum. 2. 2. Culture conditions which lead to the formation of decarboxylase activity are described. 3. 3. No other activity metabolizing 6-methylsalicylic acid can be detected in vitro with extracts containing the decarboxylase. 4. 4. The decarboxylase has a broad pH optimum between 7 and 8, and the Km for 6-methylsalicylic acid is 7 μM. Decarboxylase activity is eluted from a Bio-Gel A5m column at a position corresponding to a Stokes radius of about 30 Å. © 1969.

引用

页码：430 / &