DISCONTINUOUS UNDERCONDENSATION OF CENTROMERIC HETEROCHROMATIN IN MOUSE CHROMOSOMES - EVIDENCE IN HOECHST 33258-TREATED CELLS

被引：9

作者：

DELATORRE, J

GOYANES, V

GOSALVEZ, J

机构：

[1] UNIV AUTONOMA MADRID,FAC CIENCIAS,DEPT BIOL,UNIDAD GENET C-XV,E-28049 MADRID,SPAIN

[2] HOSP JUAN CANALEJO,CTR MATERNO INFANTIL TERESA HERRERA,SECC GENET,LA CORUNA,SPAIN

来源：

CYTOGENETICS AND CELL GENETICS | 1990年 / 54卷 / 1-2期

关键词：

D O I：

10.1159/000132954

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Mouse chromosomes From the L929 cell line have been treated with Hoechst 33258 to induce undercondensation of centromeric heterochromatin. The morphological changes induced by this fiuorochrome were analyzed in electron micrographs of whole-mounted chromosomes. Results show that the condensation inhibition of centromeric heterochromatin caused by Hoechst 33258 is not produced homogeneously and suggest compositional differences within an individual centromere. © 1990 S. Karger AG, Basel.

引用

页码：55 / 57

页数：3

共 13 条

[1]

CONINGS DE, 1975, CHROMOSOMA, V52, P229

[2] PATTERNS OF HETEROCHROMATIN REPLICATION AND CONDENSATION CORRELATE IN RAT KANGAROO PTK2 CELLS [J].

GOITEIN, R ;

HIRSCHBERG, J ;

MARCUS, M ;

SPERLING, K .

CYTOGENETICS AND CELL GENETICS, 1984, 38 (02) :116-121

[3]

GROPP A, 1973, NOBEL S, V23, P300

[4] STAINING OF CONSTITUTIVE HETEROCHROMATIN IN MAMMALIAN CHROMOSOMES WITH A NEW FLUOROCHROME [J].

HILWIG, I ;

GROPP, A .

EXPERIMENTAL CELL RESEARCH, 1972, 75 (01) :122-&

[5] DECONDENSATION OF CONSTITUTIVE HETEROCHROMATIN IN L-CELL CHROMOSOMES BY A BENZIMIDAZOLE COMPOUND (33258-HOECHST) [J].

HILWIG, I ;

GROPP, A .

EXPERIMENTAL CELL RESEARCH, 1973, 81 (02) :474-477

[6]

Holmquist G., 1988, CHROMOSOMES CHROMATI, P75

[7] OPTICAL STUDIES OF INTERACTION OF 33258 HOECHST WITH DNA, CHROMATIN, AND METAPHASE CHROMOSOMES [J].

LATT, SA ;

WOHLLEB, JC .

CHROMOSOMA, 1975, 52 (04) :297-316

[8] PREPARATION OF CENTROMERIC HETEROCHROMATIN BY RESTRICTION ENDONUCLEASE DIGESTION OF MOUSE L929 CELLS [J].

LICA, L ;

HAMKALO, B .

CHROMOSOMA, 1983, 88 (01) :42-49

[9] PATTERN OF CONDENSATION OF MOUSE AND CHINESE-HAMSTER CHROMOSOMES IN G2 AND MITOSIS OF 33258-HOECHST-TREATED CELLS [J].

MARCUS, M ;

NIELSEN, K ;

GOITEIN, R ;

GROPP, A .

EXPERIMENTAL CELL RESEARCH, 1979, 122 (01) :191-201

[10] ASYMMETRIC DECONDENSATION OF L CELL HETEROCHROMATIN BY HOECHST 33258 [J].

MATSUKUMA, S ;

UTAKOJI, T .

EXPERIMENTAL CELL RESEARCH, 1978, 113 (02) :453-455

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