MOLECULAR ANALYSIS OF A LEPTOSPIRA-BORGPETERSENII GENE ENCODING AN ENDOFLAGELLAR SUBUNIT PROTEIN

被引：24

作者：

MITCHISON, M ^{[1
]}

ROOD, JI ^{[1
]}

FAINE, S ^{[1
]}

ADLER, B ^{[1
]}

机构：

[1] MONASH UNIV,DEPT MICROBIOL,CLAYTON,VIC 3168,AUSTRALIA

来源：

JOURNAL OF GENERAL MICROBIOLOGY | 1991年 / 137卷

关键词：

INTERROGANS SEROVAR HARDJO; NUCLEOTIDE-SEQUENCE; TREPONEMA-PALLIDUM; ESCHERICHIA-COLI; FLAGELLIN GENE; GEL-ELECTROPHORESIS; CLONING; IDENTIFICATION; EXPRESSION; ANTIGENS;

D O I：

10.1099/00221287-137-7-1529

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A flagellin gene, flaB, from Leptospira borgpetersenii (formerly L. interrogans) serovar hardjo was cloned and expressed in Escherichia coli. Expression of the 32 kDa FlaB protein was dependent upon the lacZ promoter from pUC18. Nucleotide sequence data showed an open reading frame encoding 283 amino acid residues, corresponding to a protein of molecular mass 31.3 kDa. The G + C content of the flaB gene was 54.7 mol %. Comparison of the deduced FlaB amino acid sequence with flagellins from other bacteria revealed a high level of identity with the Treponema pallidum FlaB proteins.

引用

页码：1529 / 1536

页数：8

共 40 条

[11] GENOMIC ORGANIZATION AND EXPRESSION OF CAMPYLOBACTER FLAGELLIN GENES
GUERRY, P
LOGAN, SM
THORNTON, S
TRUST, TJ
[J]. JOURNAL OF BACTERIOLOGY, 1990, 172 (04) : 1853 - 1860
[12] CLONING AND NUCLEOTIDE-SEQUENCE OF A FLAGELLIN-CODING GENE (HAG) FROM SERRATIA-MARCESCENS 274
HARSHEY, RM
ESTEPA, G
YANAGI, H
[J]. GENE, 1989, 79 (01) : 1 - 8
[13] HEIN J, 1989, MOL BIOL EVOL, V6, P649
[14] HEIN J, 1989, MOL BIOL EVOL, V6, P669
[15] CODON USAGE AND TRANSFER-RNA CONTENTS - ORGANISM-SPECIFIC CODON-CHOICE PATTERNS IN REFERENCE TO THE ISOACCEPTOR CONTENTS
IKEMURA, T
OZEKI, H
[J]. COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1982, 47 : 1087 - 1097
[16] MOLECULAR-CLONING AND DNA-SEQUENCE ANALYSIS OF THE 37-KILODALTON ENDOFLAGELLAR SHEATH PROTEIN GENE OF TREPONEMA-PALLIDUM
ISAACS, RD
HANKE, JH
GUZMANVERDUZCO, LM
NEWPORT, G
AGABIAN, N
NORGARD, MV
LUKEHART, SA
RADOLF, JD
[J]. INFECTION AND IMMUNITY, 1989, 57 (11) : 3403 - 3411
[17] CULTIVATION OF PARASITIC LEPTOSPIRES - EFFECT OF PYRUVATE
JOHNSON, RC
WALBY, J
HENRY, RA
AURAN, NE
[J]. APPLIED MICROBIOLOGY, 1973, 26 (01) : 118 - 119
[18] JOYS TM, 1985, J BIOL CHEM, V260, P5758
[19] IDENTIFICATION OF LEPTOSPIRAL FLAGELLAR ANTIGENS BY GEL-ELECTROPHORESIS AND IMMUNOBLOTTING
KELSON, JS
ADLER, B
CHAPMAN, AJ
FAINE, S
[J]. JOURNAL OF MEDICAL MICROBIOLOGY, 1988, 26 (01) : 47 - 53
[20] NUCLEOTIDE-SEQUENCE OF THE HAG GENE ENCODING FLAGELLIN OF ESCHERICHIA-COLI
KUWAJIMA, G
ASAKA, JI
FUJIWARA, T
FUJIWARA, T
NODE, K
KONDO, E
[J]. JOURNAL OF BACTERIOLOGY, 1986, 168 (03) : 1479 - 1483

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