IMMUNOCHROMATOGRAPHIC ANALYSIS OF PROTEINS IDENTIFICATION, CHARACTERIZATION AND PURITY DETERMINATION

被引：18

作者：

RIGGIN, A ^{[1
]}

SPORTSMAN, JR ^{[1
]}

REGNIER, FE ^{[1
]}

机构：

[1] PURDUE UNIV,DEPT CHEM,W LAFAYETTE,IN 47907

来源：

JOURNAL OF CHROMATOGRAPHY | 1993年 / 632卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(93)80023-2

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Antibodies specific to a protein and its structural variants were immobilized on a high-performance Protein G column. This column recognized and selectively subtracted specific molecules from a sample. When a size-exclusion column was coupled with this high-performance affinity column, a comparison between the elution profile before and after the antibody immobilization was used to study antigen components present in the sample. Various human growth hormone structural variants and aggregates were studied using this approach. The technique is simple, fast and does not involve the usage of radioactive material.

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页码：37 / 44

页数：8

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