MORPHOLOGICAL EFFECTS, RATE OF INCORPORATION, AND THE ENZYMATIC ACTION OF BOTULINUM ADP-RIBOSYLTRANSFERASE, KNOWN AS C3 EXOENZYME, ON HUMAN NEUROBLASTOMA GOTO CELLS

被引：13

作者：

KAMATA, Y ^{[1
]}

NISHIKI, T ^{[1
]}

MATSUMURA, K ^{[1
]}

HIROI, T ^{[1
]}

KOZAKI, S ^{[1
]}

机构：

[1] UNIV OSAKA PREFECTURE,COLL AGR,DEPT VET SCI,SAKAI,OSAKA 593,JAPAN

来源：

MICROBIOLOGY AND IMMUNOLOGY | 1994年 / 38卷 / 06期

关键词：

BOTULINUM ADP-RIBOSYLTRANSFERASE; NEUROBLASTOMA CELLS; NEURITE FORMATION; INCORPORATION;

D O I：

10.1111/j.1348-0421.1994.tb01802.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The susceptibility of various lines of cultured cells to botulinum ADP-ribosyltransferase, known as C3 exoenzyme, was examined. Human neuroblastoma GOTO cells were most sensitive. The C3 exoenzyme caused a change in cell shape that involved extension of neurites. The exoenzyme evoked the outgrowth of neurites from chick ganglion as effectively as nerve growth factor, suggesting that C3 exoenzyme possesses neurotropic activity. Experiments with I-125-labeled enzyme revealed that C3 exoenzyme was rapidly incorporated into cells but the number of incorporated enzyme molecules was small. Once C3 exoenzyme had been incorporated, ADP-ribosylation of the substrate (Rho protein) in GOTO cells occurred immediately and rapidly reached a maximum level. However, some of Rho proteins remained unmodified even after induction of the change in morphology. These findings suggest that ADP-ribosylation by C3 exoenzyme is directly associated with the differentiation of GOTO cells but that other events may also participate in this process.

引用

页码：421 / 428

页数：8