NITROGENASE SYSTEM FROM AZOTOBACTER ACTIVATION ENERGY AND DIVALENT CATION REQUIREMENT

被引：67

作者：

BURNS, RC

机构：

[1] Charles F. Kettering Research Laboratory, Yellow Springs, OH

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1969年 / 171卷 / 02期

关键词：

D O I：

10.1016/0005-2744(69)90158-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. 1. The Arrhenius plot for nitrogenase from Azotobacter vinelandii is biphasic with an inflection at about 21°; the activation energy was calculated to be 14 600 cal/mole above this temperature and 39 000 cal/mole below. 2. 2. Values of 9 · 10-3 M and 3 · 10-4 M were determined for the Michaelis constants for hydrosulfite and for ATP, respetively, both above and below 21°. 3. 3. Mn2+, Co2+, Fe2+, and NI2+ (in that order of effectiveness) replaced Mg2+ in supporting nitrogenase activity; Cu2+ and Zn2+ were inhibitory, while Ca2+ was without effect. © 1969.

引用

页码：253 / &

共 17 条

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[14] HARDY RWF, 1968, ANNU REV BIOCHEM, V37, P331
[15] REDUCTANT-DEPENDENT ADENOSINE TRIPHOSPHATASE OF NITROGEN-FIXING EXTRACTS OF AZOTOBACTER VINELANDII
HARDY, RWF
KNIGHT, E
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[16] COMPONENTS OF CELL-FREE EXTRACTS OF CLOSTRIDIUM PASTEURIANUM REQUIRED FOR ATP-DEPENDENT H2 EVOLUTION FROM DITHIONITE AND FOR N2 FIXATION
MORTENSO.LE
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1966, 127 (01) : 18 - &
[17] SANPIETRO A, 1965, S NONHEME IRON PR ED, P261

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