THE DNA-SEQUENCE AND STRUCTURAL ORGANIZATION OF THE GC2 PLASMID FROM THE RED ALGA GRACILARIA-CHILENSIS

被引:14
作者
VILLEMUR, R [1 ]
机构
[1] NATL RES COUNCIL CANADA, ATLANTIC REG LAB, ATLANT RES LAB, HALIFAX B3H 3Z1, NS, CANADA
关键词
Gracilaria; inverted and direct repeat; plasmid; Rhodophyta;
D O I
10.1007/BF00036910
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The complete DNA sequence of the circular GC2 plasmid from the red alga Gracilaria chilensis was obtained. It contains 3827 bp and has a base composition of 75% A+T nucleotides. The sequence revealed that GC2 has two inverted repeats, each of 290 nucleotides, that border four long direct tandem repeats of 216 nucleotides. Five short, direct, tandem repeats of 21-22 nucleotides were also found in the plasmid. The plasmid sequence has one major open reading frame that could encode a 411 amino acid polypeptide. Finally, the GC2 plasmid is transcriptionally active. © 1990 Kluwer Academic Publishers.
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页码:237 / 243
页数:7
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