CLONING AND CHARACTERIZATION OF A MURINE MACROPHAGE LIPOXYGENASE

被引：42

作者：

FREIREMOAR, J ^{[1
]}

ALAVINASSAB, A ^{[1
]}

NG, M ^{[1
]}

MULKINS, M ^{[1
]}

SIGAL, E ^{[1
]}

机构：

[1] SYNTEX INC,DISCOVERY RES,INST BIOCHEM & CELLULAR BIOL,DEPT LIPID BIOCHEM & VASC BIOL,PALO ALTO,CA 94304

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM | 1995年 / 1254卷 / 01期

关键词：

INFLAMMATION; ARACHIDONIC ACID; ATHEROSCLEROSIS;

D O I：

10.1016/0005-2760(94)00199-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated a murine macrophage cDNA encoding a 12-lipoxygenase, that represents the homolog of the human 15-lipoxygenase. The predicted amino acid sequence of this lipoxygenase is highly similar to the rat 12-lipoxygenase isolated from brain and human 15-lipoxygenase. The recombinant enzyme expressed in Cos-7 cells oxidizes arachidonic acid to 12- and 15-HETE with a profile similar to that obtained from peritoneal macrophages. A polyclonal antibody generated against a putative peptide recognizes a 75 kDa protein in cell extracts from mouse peritoneal macrophages and transfected Cos-7 cells. The lipoxygenase cDNA hybridizes to a 2.5 kb mRNA present in peritoneal macrophages, lung, spleen, heart and liver. RT-PCR analysis indicates that the same lipoxygenase is expressed in mouse reticulocytes. A partial genomic clone for this lipoxygenase has also been characterized. Southern blot analysis of mouse genomic DNA indicates that this is a single copy gene.

引用

页码：112 / 116

页数：5

共 31 条

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