PROSTAGLANDIN, THROMBOXANE, AND 12-HYDROXY-5,8,10,14-EICOSATETRAENOIC ACID PRODUCTION BY IONOPHORE-STIMULATED RAT SEROSAL MAST-CELLS

被引：164

作者：

ROBERTS, LJ

LEWIS, RA

OATES, JA

AUSTEN, KF

机构：

[1] VANDERBILT UNIV,MED CTR,DEPT MED,NASHVILLE,TN 37232

[2] HARVARD UNIV,SCH MED,DEPT MED,BOSTON,MA 02115

[3] AFFILIATED HOSP CTR,ROBERT B BRIGHAM DIV,BOSTON,MA 02115

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 575卷 / 02期

基金：

美国国家卫生研究院;

关键词：

(Mast cell); 12-Hydroxy-5,8,10,14-eicosatetraenoic acid; Ionophore A23187; Prostaglandin; Thromboxane;

D O I：

10.1016/0005-2760(79)90020-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Production of several metabolites of arachidonic acid by purified rat serosal mast cells in response to stimulation with the ionophore A23187 was assessed by stable isotope dilution assay using gas chromatography-mass spectrometry. Compounds quantified were prostaglandins D2, E2, F2α, 6-keto-Fα, thromboxane B2, and 12-hydroxy-5,8,10,14-eicosatetraenoic acid. Mast cells incubated at 37°C for 30 min without ionophore produced measurable quantities of all metabolites assayed. 4 μm A23187 resulted in substantial increased synthesis of all metabolites compared to control cells. Of the metabolites quantified, prostaglandin D2 and prostacyclin were the major products derived from arachidonic acid in ionophore-stimulated rat mast cells. © 1979.

引用

页码：185 / 192

页数：8