RETENTION AND ENANTIOSELECTIVE PROPERTIES OF RACEMIC COMPOUNDS ON MODIFIED OVOMUCOID COLUMNS .2. REACTION WITH GLYCERALDEHYDE, FORMALDEHYDE AND GLUTARIC ANHYDRIDE

被引：8

作者：

HAGINAKA, J ^{[1
]}

MURASHIMA, T ^{[1
]}

SEYAMA, C ^{[1
]}

FUJIMA, H ^{[1
]}

WADA, H ^{[1
]}

机构：

[1] SHINWA CHEM IND,FUSHIMI KU,KYOTO 612,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY | 1993年 / 631卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(93)80519-E

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Modified ovomucoid (OVM) columns were prepared by reaction with glyceraldehyde, formaldehyde and glutaric anhydride. The retention and enantioselective properties of racemic compounds on these modified OVM columns were compared with those on an unmodified OVM column. The retentions of racemic compounds on the modified OVM columns were lower than or approximately equal to those on the unmodified OVM column (except for bask compounds on the OVM column reacted with glutaric anhydride). The modified OVM columns gave lower or approximately equal enantioselectivities than the unmodified OVM column for acidic and uncharged compounds, whereas the modified OVM columns gave higher enantioselectivity for basic compounds. These differences may be mainly attributed to changes in protein conformation, especially changes in chiral recognition site(s) as a result of modification. The results reveal that modification of OVM proteins may be effective for the chiral separation of basic compounds on an OVM column.

引用

页码：183 / 190

页数：8

共 8 条

[1] RETENTION AND ENANTIOSELECTIVITY OF RACEMIC SOLUTES ON A MODIFIED OVOMUCOID-BONDED COLUMN .1. CROSS-LINKING WITH GLUTARALDEHYDE [J].