HT29-18-C1 INTESTINAL-CELLS - A NEW MODEL FOR STUDYING THE EPITHELIAL TRANSPORT OF DRUGS

被引：17

作者：

WILS, P

LEGRAIN, S

FRENOIS, E

SCHERMAN, D

机构：

[1] UMR 133 CNRS, RPR, Centre de Recherche de Vitry-Alfortville, Vitry-sur-Seine

[2] Drug Safety Department, Rhône-Poulenc Rorer, Centre de Recherche de Vitry-Alfortville, Vitry-sur-Seine

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1177卷 / 02期

关键词：

INTESTINAL EPITHELIUM; DRUG TRANSPORT; HT29; PARTITION COEFFICIENT;

D O I：

10.1016/0167-4889(93)90032-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A polarized differentiated subclone of HT29, a human colon carcinoma cell line, was used to measure the passage of drugs across the intestinal epithelium. These cells, HT29-18-C1, when grown on permeable filters, formed tight monolayers of high electrical resistance (> 400 OMEGA cm2). Electron micrographs revealed the presence of numerous apical microvilli and well developed junctional complexes. The transepithelial passage of various drugs was studied: the transepithelial permeability coefficients ranged from 0.8 . 10(-6) cm/s for fluorescein, to 4.8 . 10(-5) cm/s for the most lipophilic molecule, testosterone. For the five compounds tested, there was a coarse correlation between the apparent transepithelial permeability coefficient and the octanol/buffer distribution coefficient. The HT29-18-C1 subclone thus represents a new in-vitro model for studying the intestinal absorption of drugs.

引用

页码：134 / 138

页数：5

共 19 条

[11] ABSORPTIVE AND MUCUS-SECRETING SUBCLONES ISOLATED FROM A MULTIPOTENT INTESTINAL-CELL LINE (HT-29) PROVIDE NEW MODELS FOR CELL POLARITY AND TERMINAL DIFFERENTIATION [J].