CLONING AND SEQUENCING OF THE LEISHMANIA-MAJOR ACTIN-ENCODING GENE

被引：13

作者：

DEARRUDA, MV

MATSUDAIRA, P

机构：

[1] MIT,WHITEHEAD INST BIOMED RES,CAMBRIDGE,MA 02142

[2] MIT,DEPT BIOL,CAMBRIDGE,MA 02142

来源：

GENE | 1994年 / 139卷 / 01期

关键词：

CYTOSKELETON; POLYMERASE CHAIN REACTION; RECOMBINANT DNA; SEQUENCE HOMOLOGY; TRYPANOSOMATIDS;

D O I：

10.1016/0378-1119(94)90534-7

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We report the cloning and characterization of the genomic sequence of the actin (Act)-encoding gene (net) from Leishmania major. Restriction maps of two genomic clones, as well as genomic Southern analysis strongly suggest that the net of L. major is a single-copy gene. A single 1.6-kb transcript is detected in Northern blots. The deduced aminoacid sequence shows 68-89% identity with Act sequences from other eukaryotes.

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页码：123 / 125

页数：3

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