AN IMMUNOBLOT TECHNIQUE FOR IDENTIFICATION OF ALLERGENS OF THE AUSTRALIAN PARALYSIS TICK IXODES-HOLOCYCLUS

被引：7

作者：

GAUCI, M

STONE, BF

THONG, YH

机构：

[1] CSIRO,DIV TROP ANIM PROD,LONG POCKET LABS,PRIVATE BAG 3,INDOOROOPILLY,QLD 4068,AUSTRALIA

[2] UNIV QUEENSLAND,MATER CHILDRENS HOSP,DEPT CHILD HLTH,IMMUNOBIOL LAB,BRISBANE,QLD 4101,AUSTRALIA

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 126卷 / 01期

关键词：

Allergen; Blocking agent; Ixodes holocyclus; Non-specific binding; Western blot;

D O I：

10.1016/0022-1759(90)90010-S

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The blocking conditions appropriate for Western blot identification of allergens of the Australian paralysis tick, Ixodes holocyclus, have been investigated. The results suggest that human serum is a potent blocking agent which may have wide application in the immunodetection of antigens. © 1990.

引用

页码：51 / 55

页数：5

共 20 条

[1] CHARACTERIZATION OF ALLERGENS AND PATIENT SERA BY A NITROCELLULOSE IMMUNOPRINT TECHNIQUE [J].

BENGTSSON, A ;

ROLFSEN, W ;

EINARSSON, R .

INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY, 1985, 78 (02) :139-144

[2]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[3] IDENTIFICATION OF PARIETARIA-JUDAICA POLLEN ALLERGENS [J].

FORD, SA ;

BALDO, BA ;

GERACI, D ;

BASS, D .

INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY, 1986, 79 (02) :120-126

[4] IDENTIFICATION OF ORCHARD GRASS (DACTYLIS-GLOMERATA) POLLEN ALLERGENS FOLLOWING ELECTROPHORETIC TRANSFER TO NITROCELLULOSE [J].

FORD, SA ;

TOVEY, ER ;

BALDO, BA .

INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY, 1985, 78 (01) :15-21

[5] ISOLATION AND IMMUNOLOGICAL CHARACTERIZATION OF ALLERGENS FROM SALIVARY-GLANDS OF THE AUSTRALIAN PARALYSIS TICK IXODES-HOLOCYCLUS [J].

GAUCI, M ;

STONE, BF ;

THONG, YH .

INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY, 1988, 87 (02) :208-212

[6] DETECTION IN ALLERGIC INDIVIDUALS OF IGE SPECIFIC FOR THE AUSTRALIAN PARALYSIS TICK, IXODES-HOLOCYCLUS [J].

GAUCI, M ;

STONE, BF ;

THONG, YH .

INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY, 1988, 85 (02) :190-193

[7]

HAWKES R, 1982, ANAL BIOCHEM, V119, P142, DOI 10.1016/0003-2697(82)90677-7

[8] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[9] ULTRASENSITIVE STAIN FOR PROTEINS IN POLYACRYLAMIDE GELS SHOWS REGIONAL VARIATION IN CEREBROSPINAL-FLUID PROTEINS [J].

MERRIL, CR ;

GOLDMAN, D ;

SEDMAN, SA ;

EBERT, MH .

SCIENCE, 1981, 211 (4489) :1437-1438

[10]

NEVILLE DM, 1971, J BIOL CHEM, V246, P6328

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