AN IMMUNOBLOT TECHNIQUE FOR IDENTIFICATION OF ALLERGENS OF THE AUSTRALIAN PARALYSIS TICK IXODES-HOLOCYCLUS

被引:7
作者
GAUCI, M
STONE, BF
THONG, YH
机构
[1] CSIRO,DIV TROP ANIM PROD,LONG POCKET LABS,PRIVATE BAG 3,INDOOROOPILLY,QLD 4068,AUSTRALIA
[2] UNIV QUEENSLAND,MATER CHILDRENS HOSP,DEPT CHILD HLTH,IMMUNOBIOL LAB,BRISBANE,QLD 4101,AUSTRALIA
关键词
Allergen; Blocking agent; Ixodes holocyclus; Non-specific binding; Western blot;
D O I
10.1016/0022-1759(90)90010-S
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The blocking conditions appropriate for Western blot identification of allergens of the Australian paralysis tick, Ixodes holocyclus, have been investigated. The results suggest that human serum is a potent blocking agent which may have wide application in the immunodetection of antigens. © 1990.
引用
收藏
页码:51 / 55
页数:5
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