MURINE PNEUMOCYSTIS-CARINII ADHERENCE TO VERTICAL MONOLAYERS OF CULTURED MINK LUNG-CELLS (MICL1)

被引：1

作者：

GARNER, RE ^{[1
]}

WALKER, AN ^{[1
]}

HORST, MN ^{[1
]}

机构：

[1] MERCER UNIV,SCH MED,DEPT PATHOL,MACON,GA 31207

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1992年 / 30卷 / 09期

关键词：

D O I：

10.1128/JCM.30.9.2467-2470.1992

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We describe a method for adherence and culture of murine Pneumocystis carinii in mink lung cells (MiCl1) grown on vertical supports. The vertical cultures were infected with P. carinii; the surrounding medium and inoculum were stirred to ensure circulation and contact with MiCl1 cells. When compared with conventional horizontal culture, the vertical method offers a more suitable system for assessing P. carinii adherence. This approach has proved suitable for quantitative evaluation of P. carinii adherence to MiCl1 cells in the presence of inhibitors.

引用

页码：2467 / 2470

页数：4

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