BIOSYNTHESIS OF CAERULOMYCIN-A IN STREPTOMYCES-CAERULEUS - INCORPORATION OF C-14-LABELED AND C-13-LABELED PRECURSORS AND ANALYSES OF LABELING PATTERNS BY C-13 NMR

被引：20

作者：

MCINNES, AG ^{[1
]}

SMITH, DG ^{[1
]}

WALTER, JA ^{[1
]}

VINING, LC ^{[1
]}

WRIGHT, JLC ^{[1
]}

机构：

[1] DALHOUSIE UNIV, HALIFAX B3H 4J1, NS, CANADA

来源：

CANADIAN JOURNAL OF CHEMISTRY | 1979年 / 57卷 / 24期

关键词：

D O I：

10.1139/v79-524

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

13C NMR spectroscopy of caerulomycin A (1) produced by cultures of S. caeruleus showed that [1-13C]acetate labels C-2, C-4 and C-4'', while [1,2-13C]acetate enriches these carbons plus C-2'', C-3, C-3'', C-5'' and C-6''. Acetate is incorporated with little dilution at C-3 and C-4 in the substituted ring of 1, whereas C-2, and C-2'' to C-6'' of the unsubstituted ring are assembled from lysine via the symmetrical intermediate 2S,6S-diaminopimelic acid. The methoxyl carbon incorporates label from DL-[3-13C]serine, but this precursor does not enrich C-5, C-6 or C-7 of the substituted ring, and the origins of these carbons remain undetermined.

引用

页码：3200 / 3204

页数：5