CONTINUOUS PRODUCTION OF THERMOSTABLE ALPHA-AMYLASE BY IMMOBILIZED BACILLUS CELLS IN A FLUIDIZED-BED REACTOR

被引：15

作者：

RAMAKRISHNA, SV

JAMUNA, R

EMERY, AN

机构：

[1] PENN STATE UNIV,INST BIOTECHNOL,UNIV PK,PA 16802

[2] UNIV STRATHCLYDE,DEPT BIOSCI & BIOTECHNOL,GLASGOW G1 1XW,SCOTLAND

[3] UNIV BIRMINGHAM,CTR BIOCHEM ENGN,DEPT CHEM ENGN,BIRMINGHAM B15 2TT,W MIDLANDS,ENGLAND

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1992年 / 37卷 / 03期

关键词：

IMMOBILIZATION; FLUIDIZED BED; ALPHA-AMYLASE; BACILLUS CELLS; CA-ALGINATE;

D O I：

10.1007/BF02788879

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The exponential cells of Bacillus cereus immobilized in calcium alginate and spun into microcylindrical particles were used in a fluidized-bed reactor for continuous synthesis of thermostable alpha-amylase. The reactor was operated over a period of 30 d with a dilution rate of 0.33 h-1, producing 1000-1200 U/mL of enzyme. The productivity of the reactor was in the range of 330-396 kU/h. A 20-fold increase in the productivity with respect to batch fermentation with free cells was attained.

引用

页码：275 / 282

页数：8

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